Protein Info for CSW01_06750 in Vibrio cholerae E7946 ATCC 55056

Annotation: 2-methylaconitate cis-trans isomerase PrpF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 316 to 331 (16 residues), see Phobius details TIGR02334: probable AcnD-accessory protein PrpF" amino acids 5 to 391 (387 residues), 733.2 bits, see alignment E=3.1e-225 PF04303: PrpF" amino acids 7 to 389 (383 residues), 630.5 bits, see alignment E=4.2e-194

Best Hits

Swiss-Prot: 74% identical to PRPF_CUPNE: 2-methyl-aconitate isomerase from Cupriavidus necator

KEGG orthology group: K09788, hypothetical protein (inferred from 100% identity to vch:VC1339)

Predicted SEED Role

"2-methylaconitate isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (400 amino acids)

>CSW01_06750 2-methylaconitate cis-trans isomerase PrpF (Vibrio cholerae E7946 ATCC 55056)
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQID
GMGGATSSTSKTVIVSASSRTDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAG
LIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQECGEFELDGVTFAAAEVQVDFV
DPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDL
LVRALSMGKLHHAMMGTAAVAIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKA
KQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE