Protein Info for CSW01_06745 in Vibrio cholerae E7946 ATCC 55056

Annotation: Fe/S-dependent 2-methylisocitrate dehydratase AcnD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 867 (866 residues), 1863.6 bits, E=0
• TIGR01341: aconitate hydratase 1
  • amino acids 34 to 864 (831 residues), 963.7 bits, E=7.4e-294
• PF00330: Aconitase
  • amino acids 70 to 542 (473 residues), 459 bits, E=2.2e-141
• PF00694: Aconitase_C
  • amino acids 665 to 796 (132 residues), 102.3 bits, E=2.7e-33

Best Hits

Swiss-Prot: 79% identical to ACNA_CUPNE: Aconitate hydratase A (acnM) from Cupriavidus necator

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 81% identity to avn:Avin_23230)

MetaCyc: 75% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• mixed acid fermentation (15/16 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glyoxylate cycle (6/6 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle III (animals) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• reductive TCA cycle I (8/11 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Protein Sequence (868 amino acids)

>CSW01_06745 Fe/S-dependent 2-methylisocitrate dehydratase AcnD (Vibrio cholerae E7946 ATCC 55056)
MNSLYRKALSPSPAQSQVDFFDTRAAVEALKPGAYQTLPYTARILAENLVRRCPPEQLSE
SLLQIIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEQGGDPAQVNPVVETQL
IVDHSLAVEYSGCDPDAFEKNRAVEDRRNEDRFHFIEWCKTAFKNVSVIPAGNGIMHQIN
LEKMSPVIQVKEGVAFPDSCVGTDSHTPHVDALGVLAIGVGGLEAETVMLGRPSMMRLPD
IVGVKLTGARQPGITATDIVLALTEFLRRERVVSAYLEFFGEGAKALTIGDRATISNMTP
EYGATAGMFYIDEQTIQYLKLTGREPEQVALVESYAKAAGLWADSLEHAEYERVLEFDLS
SVERNLAGPSNPHRRLPTKDLSARGIAIPAQQREAQQAEGLMPDGAVIIAAITSCTNTSN
PRNVVAAGLLAKKANQLGLKRQPWVKTSFAPGSKVAKLYLQEAGLLSELEQLGFGIVAYA
CTTCNGMSGALDPAIQQEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAI
AGTMRFDIERDALGHDAQGKPIYLNHLWPSDEEIDAVVGRAVKPEQFKQIYIQMFKLDET
QSASSPLYDWRPMSTYIRRPPYWEGALAAPRTLKAMRPLAILGDNITTDHLSPSNAILAS
SAAGEYLTKMGVPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKENGQIKQGSLARIE
PEGKVTRMWEAIETYMNRKQPLIVIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIH
RTNLVGMGVLPLEFKPGVNRHSLALDGTELFDVVGEIRPGADLALVVTRQNGEKLDVAVT
CRLDTADEVHVYQAGGVLQRFAQDFLAQ