Protein Info for CSW01_06085 in Vibrio cholerae E7946 ATCC 55056

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR01228: urocanate hydratase" amino acids 15 to 556 (542 residues), 973.2 bits, see alignment E=1.8e-297 PF17391: Urocanase_N" amino acids 17 to 143 (127 residues), 209.7 bits, see alignment E=1.6e-66 PF01175: Urocanase" amino acids 146 to 351 (206 residues), 291.7 bits, see alignment E=4.9e-91 PF17392: Urocanase_C" amino acids 354 to 548 (195 residues), 306.5 bits, see alignment E=9.9e-96

Best Hits

Swiss-Prot: 100% identical to HUTU_VIBCH: Urocanate hydratase (hutU) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to vcm:VCM66_1158)

MetaCyc: 78% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>CSW01_06085 urocanate hydratase (Vibrio cholerae E7946 ATCC 55056)
MTQSSAQGTRLDTQRTIRAPRGTQLRAKSWLTEAPLRMLMNNLDPDVAEHPHALVVYGGI
GRAARNWECFDKIVEVLERLEDDQTLLVQSGKPVGVFPTHKNAPRVLIANSNLVPHWANW
EHFNELDKQGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAVAKKHFNGDAKGRWVLTGGL
GGMGGAQPLAATMAGFSMIAVECDESRIDYRLRTGYVDKKANTLDEALAMIADTDRPISV
GLLGNAADIFPELVKRNITPDVVTDQTSAHDPLNGYLPLGWSMEKAAQMRQQNEAEVVKA
AKASMAIQVRAMLDLQTRGAATLDYGNNIRQMALEEGVANAFDFPGFVPAYIRPLFCEGI
GPFRWAALSGDPEDIYKTDQKVKELIPDNPHLHNWLDMARERIHFQGLPARICWVGLKDR
ARLGLAFNEMVKNGELKAPIVIGRDHLDSGSVASPNRETEGMLDGSDAVSDWPLLNALLN
TAGGATWVSLHHGGGVGMGFSQHSGMVICCDGSDDAAERIARVLHNDPATGVMRHADAGY
EIAKRCAQQQKLDLPMLNAELAKLK