Protein Info for CSW01_05910 in Vibrio cholerae E7946 ATCC 55056

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 582 (582 residues), 877.5 bits, see alignment E=2e-268 PF01336: tRNA_anti-codon" amino acids 18 to 102 (85 residues), 62.3 bits, see alignment E=5e-21 PF00152: tRNA-synt_2" amino acids 117 to 557 (441 residues), 408.5 bits, see alignment E=2.5e-126 PF02938: GAD" amino acids 307 to 406 (100 residues), 105.8 bits, see alignment E=1.8e-34

Best Hits

Swiss-Prot: 100% identical to SYD_VIBCH: Aspartate--tRNA ligase (aspS) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to vcj:VCD_003177)

MetaCyc: 76% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (591 amino acids)

>CSW01_05910 aspartate--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVA
NQLRSEFCIKLTGEVRARPESQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQ
RLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDIETPVLTKATPEGARDYLVPS
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLK
DVDFKVFSGPANDPKGRVAALCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAG
MEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAKVVAEALGALRLKAGKELGIT
NESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVM
LLCGTENIRDVIAFPKTTAAACLMTDAPSLANPAALEELAIAVKLATKDKA