Protein Info for CSW01_05805 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 756 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 737 (735 residues), 1101.3 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 47.4 bits, see alignment 1e-15 PF00004: AAA" amino acids 214 to 330 (117 residues), 47.1 bits, see alignment E=2e-15 amino acids 495 to 610 (116 residues), 34.1 bits, see alignment E=2e-11 PF17871: AAA_lid_9" amino acids 354 to 457 (104 residues), 100 bits, see alignment E=4.1e-32 PF07724: AAA_2" amino acids 489 to 649 (161 residues), 199.1 bits, see alignment E=3.3e-62 PF07728: AAA_5" amino acids 494 to 610 (117 residues), 41.5 bits, see alignment E=8.1e-14 PF10431: ClpB_D2-small" amino acids 656 to 736 (81 residues), 91.1 bits, see alignment E=2.2e-29

Best Hits

Swiss-Prot: 78% identical to CLPA_ECOLI: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli (strain K12)

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 82% identity to vsa:VSAL_I2214)

MetaCyc: 78% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (756 amino acids)

>CSW01_05805 ATP-dependent Clp protease ATP-binding subunit ClpA (Vibrio cholerae E7946 ATCC 55056)
MLNKELESSLNGAFARARDKRHEFMTVEHLLLALLENDAAREALLACQADIDVLRRELDT
FIDQTTPLIPKNDETRETQPTLSFQRVLQRAVFHVQSSGRSEVTGANVLVAIFSEQESHA
AYLLKKNDISRLDIVNFISHGITKASNHSEDSSSDSFGSSDTSEEVSADERLESFATNLN
QLAKQGQIDPLIGRDKELERTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRIVEGNVP
EVIQRSVIYSLDIGSLLAGTKYRGDFEKRFKSILKQLEKEKDAILFIDEIHTIIGAGAAS
GGQVDAANLIKPLLSSGKLRCIGSTTYQEYSNIFEKERALSRRFQKIDIVEPSLDDTTKI
LMGLKTKYEAHHDVRYTNKALRAAVELSAKYINERHLPDKAIDVIDEAGARARLMPASRR
KKTVGVAEIESMVAKMARIPEKSVSSSDKDILKNLDQKMKMLVFGQDAAIDVLTEAIKLT
RAGLGAEHKPVGSFLFAGPTGVGKTEVTLQLSKLLGIELLRFDMSEYGERHSVSRLIGAP
PGYVGYDQGGLLTDAVIKHPHSVVLLDEIEKAHPDIFNLLLQVMDNGTLTDNNGRKADFR
NVILVMTTNAGVAETVKKSIGLIQQDNSHDAMSEIKKVFTPEFRNRLDHIIWFNSLDERV
IHQVVDKFIVELQAQLDARGVSLEVSEDARHWLAVKGYDREMGARPMGRVIQEQLKKPLA
NELLFGSLVDGGTVKVTLSDDRLAFEYFGTREEVVH