Protein Info for CSW01_05785 in Vibrio cholerae E7946 ATCC 55056

Annotation: 23S rRNA pseudouridine synthase E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 PF00849: PseudoU_synth_2" amino acids 66 to 210 (145 residues), 64.7 bits, see alignment E=5.7e-22 TIGR00093: pseudouridine synthase" amino acids 70 to 240 (171 residues), 196.6 bits, see alignment E=1.1e-62

Best Hits

Swiss-Prot: 100% identical to RLUE_VIBCH: Ribosomal large subunit pseudouridine synthase E (rluE) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 100% identity to vco:VC0395_A0710)

MetaCyc: 64% identical to 23S rRNA pseudouridine2457 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11834 [EC: 5.4.99.20]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (241 amino acids)

>CSW01_05785 23S rRNA pseudouridine synthase E (Vibrio cholerae E7946 ATCC 55056)
MSSRLSSDATSSSSDQRKSHFKQRAAKNAGHPPTRANRKSVANKKKNATQTALSPKRPLS
PAERKVILFNKPYDTLSQFTDGDGRKTLADYIPIKDVYAAGRLDRDSEGLLILTNDGILQ
ARLTQPQSKAPKTYWVQVEGSPQESDLEALRHGVTLKDGPTLPAKVDIMPEPTLWPRNPP
VRFRAAIPTTWLAITLMEGRNRQVRRMTAHIGFPTLRLIRYSMGDWNLGDLQPGEWREVT
L