Protein Info for CSW01_05715 in Vibrio cholerae E7946 ATCC 55056

Annotation: adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 12 to 456 (445 residues), 502.4 bits, E=5.2e-155
• PF00206: Lyase_1
  • amino acids 14 to 313 (300 residues), 240.3 bits, E=3.6e-75
• PF08328: ASL_C
  • amino acids 332 to 446 (115 residues), 173.3 bits, E=1.6e-55

Best Hits

Swiss-Prot: 82% identical to PUR8_ECOL6: Adenylosuccinate lyase (purB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 100% identity to vcj:VCD_003216)

MetaCyc: 82% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)
• dZTP biosynthesis (3/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Protein Sequence (456 amino acids)

>CSW01_05715 adenylosuccinate lyase (Vibrio cholerae E7946 ATCC 55056)
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFS
AEANQFLDAIAANFNEADALRIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFA
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEEYRDIPLLSRTHGQPASPSTM
GKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMP
HKVNPIDFENSEGNLGLANAVFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTS
TLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDGEAM
RQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL