Protein Info for CSW01_05650 in Vibrio cholerae E7946 ATCC 55056

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00858: 8-amino-7-oxononanoate synthase
  • amino acids 22 to 377 (356 residues), 485.7 bits, E=3.4e-150
• PF00155: Aminotran_1_2
  • amino acids 43 to 375 (333 residues), 193.4 bits, E=3.4e-61

Best Hits

Swiss-Prot: 100% identical to BIOF_VIBCH: 8-amino-7-oxononanoate synthase (bioF) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 100% identity to vch:VC1113)

MetaCyc: 40% identical to 8-amino-7-oxononanoate synthase (pimeloyl-CoA-dependent) (Bacillus subtilis subtilis 168)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]; 2.3.1.47 [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

• biotin biosynthesis I (13/15 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• biotin biosynthesis II (4/6 steps found)
• 8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
• 8-amino-7-oxononanoate biosynthesis III (1/2 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Protein Sequence (384 amino acids)

>CSW01_05650 8-amino-7-oxononanoate synthase (Vibrio cholerae E7946 ATCC 55056)
MTLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAEL
VQAWQTGLARFGAGSGASPMVTGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFT
LLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHLRQLLHEQSNSLVVTEGVFSM
DGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLA
GAAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFD
EQCRNIPGFVATQTPIKPWLLGSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITI
TAAHTQAQIRRLSEQLKHVMEKDT