Protein Info for CSW01_05100 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 32 to 415 (384 residues), 358.8 bits, E=2.5e-111
• PF08245: Mur_ligase_M
  • amino acids 54 to 196 (143 residues), 27 bits, E=4.1e-10
• PF02875: Mur_ligase_C
  • amino acids 289 to 406 (118 residues), 78.5 bits, E=8.6e-26

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to vcj:VCD_003337)

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of chorismate metabolism (51/59 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (421 amino acids)

>CSW01_05100 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Vibrio cholerae E7946 ATCC 55056)
MTQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGK
GSTCALMEAILLQAGYSVGVYSSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDIS
LSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVDHDVSVITSLALDHTDWLGND
IAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWR
SGRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTI
VLDVAHNPHSAHYLVQQLTQRYPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSG
PRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVIVVAGSFHTVGEVLEHWQKRG
E