Protein Info for CSW01_04925 in Vibrio cholerae E7946 ATCC 55056

Annotation: PTS glucose transporter subunit IIA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00358: PTS_EIIA_1
  • amino acids 22 to 146 (125 residues), 164.4 bits, E=4.8e-53
• TIGR00830: PTS system, glucose subfamily, IIA component
  • amino acids 23 to 143 (121 residues), 165.9 bits, E=1.7e-53

Best Hits

Swiss-Prot: 89% identical to PTGA_ECOL6: PTS system glucose-specific EIIA component (crr) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02777, PTS system, glucose-specific IIA component [EC: 2.7.1.69] (inferred from 99% identity to vco:VC0395_A0486)

MetaCyc: 89% identical to Enzyme IIAGlc (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-168 [EC: 2.7.1.201]; TRANS-RXN-157 [EC: 2.7.1.201, 2.7.1.199]; RXN0-17 [EC: 2.7.1.201, 2.7.1.199, 2.7.1.192]

Predicted SEED Role

"PTS system, glucose-specific IIA component (EC 2.7.1.69)" in subsystem Trehalose Uptake and Utilization (EC 2.7.1.69)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.192 or 2.7.1.199 or 2.7.1.201 or 2.7.1.69

Protein Sequence (169 amino acids)

>CSW01_04925 PTS glucose transporter subunit IIA (Vibrio cholerae E7946 ATCC 55056)
MGLFDKLKKLVSDDSANAGAIDIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPAGNKM
VAPVNGTIGKIFETNHAFSIESDDGVELFVHFGIDTVELKGEGFKRIAEEGQSVKIGDTI
IEFDLALLEEKAKSTLTPVVISNMDEIKELNKLSGSVTVGETPILRVTK