Protein Info for CSW01_04845 in Vibrio cholerae E7946 ATCC 55056

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00768: Peptidase_S11" amino acids 31 to 262 (232 residues), 321.5 bits, see alignment E=5.1e-100 PF13354: Beta-lactamase2" amino acids 44 to 194 (151 residues), 36.3 bits, see alignment E=5.8e-13 PF07943: PBP5_C" amino acids 282 to 372 (91 residues), 101.3 bits, see alignment E=4e-33

Best Hits

Swiss-Prot: 58% identical to DACA_ECO57: D-alanyl-D-alanine carboxypeptidase DacA (dacA) from Escherichia coli O157:H7

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 100% identity to vch:VC0947)

MetaCyc: 58% identical to D-alanyl-D-alanine carboxypeptidase DacA (Escherichia coli K-12 substr. MG1655)
Beta-lactamase. [EC: 3.5.2.6]; Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8 or 3.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>CSW01_04845 D-alanyl-D-alanine carboxypeptidase (Vibrio cholerae E7946 ATCC 55056)
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDT
KLSPASLTKMMTSYVIGQEVKRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSD
LNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLGMKNSHFTNSHGLDDPNLYST
PYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETVAPHKAGETFVNETIW
MGDKDTIALGVDKDTYVTLPRGQAKDLTASFVLEKQLKAPLKKGDIVGTLYYQLAGNDIA
QYPLLALEDVQEGSLFSRLWDYLVLLFKSWF