Protein Info for CSW01_04650 in Vibrio cholerae E7946 ATCC 55056

Annotation: D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 186 TIGR00213: D,D-heptose 1,7-bisphosphate phosphatase" amino acids 4 to 178 (175 residues), 263.5 bits, see alignment E=1.2e-82 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 4 to 152 (149 residues), 133.6 bits, see alignment E=8e-43 TIGR01662: HAD hydrolase, family IIIA" amino acids 5 to 154 (150 residues), 124.9 bits, see alignment E=3.8e-40 PF00702: Hydrolase" amino acids 19 to 146 (128 residues), 36.7 bits, see alignment E=1.2e-12 PF08645: PNK3P" amino acids 21 to 140 (120 residues), 30.9 bits, see alignment E=4.3e-11 PF13419: HAD_2" amino acids 103 to 148 (46 residues), 28.2 bits, see alignment E=4e-10 PF13242: Hydrolase_like" amino acids 107 to 176 (70 residues), 47.6 bits, see alignment E=2.5e-16

Best Hits

Swiss-Prot: 100% identical to GMHBB_VIBCH: D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03273, D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase [EC: 3.1.3.-] (inferred from 100% identity to vcj:VCD_003424)

MetaCyc: 62% identical to D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (Escherichia coli K-12 substr. MG1655)
RXN0-4361 [EC: 3.1.3.82]

Predicted SEED Role

"D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-)" in subsystem Capsular heptose biosynthesis or LOS core oligosaccharide biosynthesis (EC 3.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-, 3.1.3.-

Use Curated BLAST to search for 3.1.1.- or 3.1.3.- or 3.1.3.82

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (186 amino acids)

>CSW01_04650 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase (Vibrio cholerae E7946 ATCC 55056)
MAKPAVFLDRDGVINVDHGYVHDEHDFQFIEGVFEATAALQRMGYLLVLVTNQSGIARGK
FSEERFISLTQWMDWNFADNGVEFDGIYYCPHHAEHGIGQYKEECDCRKPKPGMFLSARD
FLNIDMANSVMVGDKAEDMMAAEAAGVGTKILVRTGKPITEQGEALATVVLDSIRDVPHY
LLRVKK