Protein Info for CSW01_04560 in Vibrio cholerae E7946 ATCC 55056

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 12 to 624 (613 residues), 998.6 bits, E=5.6e-305
• PF13292: DXP_synthase_N
  • amino acids 14 to 282 (269 residues), 397.8 bits, E=4.9e-123
• PF02775: TPP_enzyme_C
  • amino acids 122 to 183 (62 residues), 22.7 bits, E=1.5e-08
• PF02779: Transket_pyr
  • amino acids 318 to 479 (162 residues), 157.4 bits, E=5.9e-50
• PF02780: Transketolase_C
  • amino acids 506 to 616 (111 residues), 95.1 bits, E=6.2e-31

Best Hits

Swiss-Prot: 100% identical to DXS_VIBCM: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 100% identity to vcj:VCD_003443)

MetaCyc: 76% identical to 1-deoxy-D-xylulose-5-phosphate synthase (Escherichia coli K-12 substr. MG1655)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• isoprene biosynthesis I (9/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (8/12 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.7

Protein Sequence (626 amino acids)

>CSW01_04560 1-deoxy-D-xylulose-5-phosphate synthase (Vibrio cholerae E7946 ATCC 55056)
MTLDISKYPTLALANTPDELRSLPKEVLPKLCDELRTYLLNSVSQSSGHLASGLGTVELT
VALHYVYHTPFDHLIWDVGHQAYPHKILTGRRDQMPTIRQKDGLHPFPWREESEYDTLSV
GHSSTSISAALGMAICAGKEGKDRKVVSVIGDGAITAGMAFEAMNHAGDVHPDMLVVLND
NEMSISENVGALNNHLAQVLSGSLYTSIREGGKKVLSGIPPIKELVRRTEEHLKGMVVPG
TLFEELGFNYIGPVDGHDVLELIKTLKNMRELKGPQFLHVMTKKGKGYAPAEKDPIGYHG
VPKFDPSHHSLPKSSNTKPTFSKIFGDFLCDMAAQDPKLMAITPAMREGSGMVRFSKEYP
SQYFDVAIAEQHAVTLATGMAIAGYHPIVAIYSTFLQRGYDQLIHDVAIMNLPVMFAIDR
AGIVGADGQTHQGAFDLSYMRCIPNMLIMAPADENECRQMLYTGHQHQGPSAVRYPRGNG
MGVELESSFTALEIGKGRLMRESTACEGEKVAILSFGTLLPNALQAAEKLNATVADMRFV
KPLDEALIKQLAQTHDVLVTLEENAIAGGAGAGVIEFLMKEKQLKPVLNLGLPDQFIVQG
TQEEMHAELGLDGAGIERAIRDYLAK