Protein Info for CSW01_04130 in Vibrio cholerae E7946 ATCC 55056

Annotation: citrate lyase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 TIGR01584: citrate lyase, alpha subunit" amino acids 37 to 507 (471 residues), 784.4 bits, see alignment E=2.1e-240 PF04223: CitF" amino acids 41 to 506 (466 residues), 921.8 bits, see alignment E=4.7e-282

Best Hits

Swiss-Prot: 70% identical to CILA_ECOLI: Citrate lyase alpha chain (citF) from Escherichia coli (strain K12)

KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 100% identity to vcj:VCD_003526)

MetaCyc: 70% identical to citrate lyase alpha subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>CSW01_04130 citrate lyase subunit alpha (Vibrio cholerae E7946 ATCC 55056)
MSINTLNNPLSVLEVERYALTPYTQARAITPHLHDEQAKKSRKVKSSIREAIVDLGLKDG
MTISFHHAFRGGDKTVNLVMEVIAELGIKNLTLASSSLTSCHSPLIEHIKNGVVSKIYTS
GIRGALADSISNGLLDEPVHIHSHGGRVHLIQSGELYIDMAFIGVPCCDEFGNANGFKGK
SNCGSLGYAKVDAEHAEKVVMLTEAIVGFPNFPASITQDRVDAVVQVAEVGDPSKIGGDA
TRMTTNPRELLIAKRAAEVIEHSGYFYDGFSMQTGSGGASLAVARFLKEKMLRQDIRASF
ALGGITATMVDMHEQGLIDYLLDVQCFDSVAAGSLARNPHHLEISANEYANPSSKGAAVD
RLDVVILSALEIDTQFNVNVITGSDGVIRGASGGHCDTAAAANLSIIVAPLVRGRIPTVV
EKVTNVITPGSTIDVLVTDQGIAVNPNRPELKARFIEAQLPVVEIEALQQRAELLTGKPQ
PLQFEDKTVAFVHYRDGSIIDVIKQVKSL