Protein Info for CSW01_03575 in Vibrio cholerae E7946 ATCC 55056

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 853 (839 residues), 967 bits, see alignment E=4.8e-295 PF00133: tRNA-synt_1" amino acids 28 to 646 (619 residues), 499.6 bits, see alignment E=1.9e-153 PF09334: tRNA-synt_1g" amino acids 58 to 202 (145 residues), 41.7 bits, see alignment E=2.1e-14 PF08264: Anticodon_1" amino acids 691 to 853 (163 residues), 94.3 bits, see alignment E=2.1e-30 PF06827: zf-FPG_IleRS" amino acids 910 to 938 (29 residues), 33.4 bits, see alignment (E = 9.2e-12)

Best Hits

Swiss-Prot: 82% identical to SYI_ALIFM: Isoleucine--tRNA ligase (ileS) from Aliivibrio fischeri (strain MJ11)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 81% identity to vsa:VSAL_I0580)

MetaCyc: 68% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (949 amino acids)

>CSW01_03575 isoleucine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MSEYKDTLNLPETGFPMRGDLAKREPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYA
NGDIHIGHALNKILKDVIIKSKTLSGFDAPYIPGWDCHGLPIELMVEKKVGKPGQKVTAA
EFREKCREYAAGQVEGQKESFKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLLK
GFKPVHWCTDCGSALAEAEVEYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSI
VIWTTTPWTLPANRAVCLRADLEYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLG
FATGADLELVQFQHPFYSFTVPAILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGLEVA
NPVGSNGVYLPDTELFAGQHVFKANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTPIIF
RATPQWFVSMEQAGLREQALTAIKGVHWMPDWGQSRIEGMVAGRPEWCISRQRTWGVPIA
LFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAELLGAEDAANYEKVLDTLDVW
FDSGVTHSAVVDARQEFNGAEADMYLEGSDQHRGWFQSSLISSVAMKGKAPYKEVLTHGF
VVDGQGRKMSKSIGNVVAPQDVTNKLGADILRLWVASTDYTGEVAVSDEILKRSADAYRR
IRNTARFFLANLNGFNPTTDIIPVEDMVALDRWAVGRALAAQQEIIQAYQDYNLHAVVQR
LMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQRSCQTALFFIVEALVRWMAPIMSFTADEI
WNAMPAQQADGSARDKFVFTTEWFDGLFGLAEGEELNNAFWNDIQKVRGSVNKLLENARN
EKLIGGSLQAELVLFADDSLASKLAKLGDELRFVLLTSKAVVKPLAEKSEAAQATDIDGL
FVQVNKTEAEKCDRCWHHTPDVGTIAGHTTICGRCVSNVEGEGEVRKFA