Protein Info for CSW01_03570 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 11 to 167 (157 residues), 202.3 bits, E=6.1e-64
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 18 to 307 (290 residues), 348.8 bits, E=1.3e-108
• PF01467: CTP_transf_like
  • amino acids 19 to 170 (152 residues), 24.9 bits, E=3.2e-09
• PF01687: Flavokinase
  • amino acids 184 to 306 (123 residues), 144.9 bits, E=2.2e-46

Best Hits

Swiss-Prot: 59% identical to RIBF_ECO57: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli O157:H7

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to vcm:VCM66_0639)

MetaCyc: 59% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (1/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (18/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (322 amino acids)

>CSW01_03570 bifunctional riboflavin kinase/FMN adenylyltransferase (Vibrio cholerae E7946 ATCC 55056)
MELIRGIHNIQSRHHGCVLTIGNFDGVHLGHQQVLSQVVQRAQQMGLPSVVMTFEPQPLE
LFAKQQAPARLTRLRDKFVQLSKLGVDRLLCVNFNHHFAQLDAHTFIRDLLVTRLGVKFL
VVGDDFCFGKERQGNFALLQQAGQALDFEVVSTQSFCLQQLRVSSTAIREALAADQLSSA
SAMLGRHYSISGRVSHGQKLGRTIGFPTANIPLKRCVSPVSGVYVVEAYGLGPTPVGGVA
NIGNRPTVDGVRQQLEVHFFDFQGNLYGKQLEVALLHKLRDERKFASFEALKQQIELDAE
AARVWLRQLKGCSVDSTQQQHV