Protein Info for CSW01_03525 in Vibrio cholerae E7946 ATCC 55056

Annotation: phosphoenolpyruvate-protein phosphotransferase PtsP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 transmembrane" amino acids 139 to 157 (19 residues), see Phobius details PF13185: GAF_2" amino acids 17 to 153 (137 residues), 50.5 bits, see alignment E=7.8e-17 PF01590: GAF" amino acids 20 to 151 (132 residues), 59.4 bits, see alignment E=1.7e-19 PF13492: GAF_3" amino acids 21 to 151 (131 residues), 32.7 bits, see alignment E=2.8e-11 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 171 to 728 (558 residues), 446.2 bits, see alignment E=8e-138 PF05524: PEP-utilisers_N" amino acids 172 to 294 (123 residues), 88.3 bits, see alignment E=1.3e-28 PF00391: PEP-utilizers" amino acids 320 to 391 (72 residues), 62.2 bits, see alignment E=8.8e-21 PF02896: PEP-utilizers_C" amino acids 423 to 706 (284 residues), 324.1 bits, see alignment E=2.3e-100

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to vcm:VCM66_0630)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (748 amino acids)

>CSW01_03525 phosphoenolpyruvate-protein phosphotransferase PtsP (Vibrio cholerae E7946 ATCC 55056)
MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKF
KGDKIHIDFDEGLVGLVKRSAEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQV
LGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQGHWRLSKKQQAITGVAASSGV
AIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVENALADFRRMRKKLDGDINKEALA
IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARMSDVYLRERAQDIRE
LGQRLLFFLLNTETNQPKIDKPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAIL
SRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKTILNEYRALANEERELSRMVN
EAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT
QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLR
AMLRASAESGNLSILLPMISGAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSM
LYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYESMHPAVVMALKQIQQTCATH
QIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ
PYGREIYNMMLAYIEKHGFAGFVRAGKK