Protein Info for CSW01_03480 in Vibrio cholerae E7946 ATCC 55056

Annotation: tRNA (adenosine(37)-N6)-methyltransferase TrmM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 PF06325: PrmA" amino acids 43 to 117 (75 residues), 33 bits, see alignment E=2.3e-11 PF13847: Methyltransf_31" amino acids 44 to 164 (121 residues), 36.8 bits, see alignment E=1.6e-12 PF05175: MTS" amino acids 45 to 130 (86 residues), 62.4 bits, see alignment E=2.2e-20 PF01596: Methyltransf_3" amino acids 45 to 103 (59 residues), 22.6 bits, see alignment E=2.9e-08 PF13649: Methyltransf_25" amino acids 46 to 118 (73 residues), 47.5 bits, see alignment E=1.3e-15 PF08241: Methyltransf_11" amino acids 47 to 117 (71 residues), 30 bits, see alignment E=3.3e-10 PF08242: Methyltransf_12" amino acids 47 to 116 (70 residues), 31.5 bits, see alignment E=1.3e-10 PF01170: UPF0020" amino acids 65 to 128 (64 residues), 26.5 bits, see alignment E=2.4e-09

Best Hits

Swiss-Prot: 100% identical to TRMN6_VIBCH: tRNA1(Val) (adenine(37)-N6)-methyltransferase (VC_0661) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: None (inferred from 100% identity to vch:VC0661)

Predicted SEED Role

"tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223)" (EC 2.1.1.223)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.223

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (240 amino acids)

>CSW01_03480 tRNA (adenosine(37)-N6)-methyltransferase TrmM (Vibrio cholerae E7946 ATCC 55056)
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMC
AQRFPHAHITALDIEQTAYLAAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPY
FNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVASFILPKAEGEDFIALARQAG
LFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS