Protein Info for CSW01_03410 in Vibrio cholerae E7946 ATCC 55056

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 11 to 692 (682 residues), 1083.6 bits, see alignment E=0 PF01138: RNase_PH" amino acids 16 to 145 (130 residues), 92.4 bits, see alignment E=8.3e-30 amino acids 325 to 457 (133 residues), 94.5 bits, see alignment E=1.8e-30 PF03725: RNase_PH_C" amino acids 148 to 211 (64 residues), 57.5 bits, see alignment E=2.8e-19 amino acids 462 to 528 (67 residues), 23.5 bits, see alignment E=1.1e-08 PF03726: PNPase" amino acids 242 to 321 (80 residues), 94.8 bits, see alignment E=8.7e-31 PF00013: KH_1" amino acids 557 to 615 (59 residues), 40.4 bits, see alignment 5e-14 PF00575: S1" amino acids 620 to 691 (72 residues), 67.4 bits, see alignment E=3e-22

Best Hits

Swiss-Prot: 100% identical to PNP_VIBCH: Polyribonucleotide nucleotidyltransferase (pnp) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to vch:VC0647)

MetaCyc: 77% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (709 amino acids)

>CSW01_03410 polyribonucleotide nucleotidyltransferase (Vibrio cholerae E7946 ATCC 55056)
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPL
TVNYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVS
VNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDGQLVLNPSEKELKQSRLDLVV
AGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHD
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQ
RDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGIAAVMPSPE
EFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQ
AISAARSDISEFAPRIHTMKISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAA
TDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFVTILPGKDGLVHISQIADKRV
EKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE