Protein Info for CSW01_03390 in Vibrio cholerae E7946 ATCC 55056

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 898 PF04760: IF2_N" amino acids 1 to 51 (51 residues), 38.7 bits, see alignment 2.4e-13 amino acids 322 to 372 (51 residues), 59.1 bits, see alignment 1e-19 PF08364: IF2_assoc" amino acids 61 to 99 (39 residues), 61 bits, see alignment (E = 3.6e-20) TIGR00487: translation initiation factor IF-2" amino acids 315 to 897 (583 residues), 945.8 bits, see alignment E=1.1e-288 PF00009: GTP_EFTU" amino acids 400 to 557 (158 residues), 118.4 bits, see alignment E=1.1e-37 TIGR00231: small GTP-binding protein domain" amino acids 400 to 555 (156 residues), 110.6 bits, see alignment E=6.6e-36 PF01926: MMR_HSR1" amino acids 402 to 507 (106 residues), 35.1 bits, see alignment E=4.6e-12 PF22042: EF-G_D2" amino acids 573 to 651 (79 residues), 109.9 bits, see alignment E=1.8e-35 PF11987: IF-2" amino acids 673 to 787 (115 residues), 148.3 bits, see alignment E=3e-47 PF03144: GTP_EFTU_D2" amino acids 818 to 885 (68 residues), 36.9 bits, see alignment 1.5e-12

Best Hits

Swiss-Prot: 78% identical to IF2_ALIF1: Translation initiation factor IF-2 (infB) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 74% identity to vsa:VSAL_I0598)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (898 amino acids)

>CSW01_03390 translation initiation factor IF-2 (Vibrio cholerae E7946 ATCC 55056)
MTQITVKALSEEIGTPVDRLLEQLADAGMNKAVADHVSEDEKQKLLAHLRKEHGDATGSE
PTRLTLQRKTRSTLSVNAGGGKSKNVQVEVRKKRTYVKRSSVEDEATREAEEAAMRAAEE
QAKREAEEAAQRAAEEKAKREAEEAAKREAEAKRMAEEKAKRETQAATQPRSDEEKLKQE
AARKEAEALKRRQEEEARRKAEEESRRQLEKVRELAEKNGERWSADKETVGDMQENTDYH
VTTSRYAREAEDEADLHEEGARRRSTKANKRKMSSRDDNQERDSRPRGGKAGRKGRINKP
MSMQHGFDKTAVVAKADVVVGETIVVSELAQKMSVKATEVIKVMMKMGAMATINQVIDQE
TAQLVAEEMGHKVVLRKENELEEAILSDRDDKFEEVSRAPVVTIMGHVDHGKTSTLDYIR
RTHVASGEAGGITQHIGAYHVETPNGMITFLDTPGHAAFTAMRARGAQATDIVVLVVAAD
DGVMPQTVEAIQHAKAAGVPLIVAVNKIDKDTANPDNVKTELSQYNVMPEEWGGDNMFVH
ISAKQGTNIDGLLEAILLQAEVLELKAVKQGMASGVVIESRLDKGRGPVATVLVQSGTLR
KGDIVLCGQEYGRVRAMRDEVGNEVEEAGPSIPVEILGLSGVPAAGDEATVVRDERKARE
VANYRAGKFREVKLARQQKSKLENMFSNMTAGDVAELNIVLKADVQGSVEAIADSLTKLS
TDEVKVNIVGSGVGGITETDAVLAAASNAIVVGFNVRADASARRMIEAENIDLRYYSIIY
QLIDEVKQAMSGMLSPEFKQEIIGLAEVRDVFKSPKLGAIAGCMVTEGVIKRNAPIRVLR
DNVVIYEGELESLRRFKDDVAEVKNGYECGIGVKNYNDVRVGDQIEVFETIEIQRTID