Protein Info for CSW01_03175 in Vibrio cholerae E7946 ATCC 55056

Annotation: penicillin-binding protein 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 transmembrane" amino acids 32 to 54 (23 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 35 to 758 (724 residues), 1184.2 bits, see alignment E=0 PF14814: UB2H" amino acids 80 to 164 (85 residues), 95.9 bits, see alignment E=1.8e-31 PF00912: Transgly" amino acids 176 to 347 (172 residues), 196.5 bits, see alignment E=3.9e-62 TIGR02074: penicillin-binding protein, 1A family" amino acids 180 to 714 (535 residues), 524.2 bits, see alignment E=4.2e-161 PF00905: Transpeptidase" amino acids 439 to 678 (240 residues), 82.7 bits, see alignment E=4.1e-27

Best Hits

Swiss-Prot: 100% identical to PBPB_VIBCH: Penicillin-binding protein 1B (mrcB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 100% identity to vcm:VCM66_0560)

MetaCyc: 52% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (777 amino acids)

>CSW01_03175 penicillin-binding protein 1B (Vibrio cholerae E7946 ATCC 55056)
MTRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQ
RFEGQLFDLPTVVYARILTLEPGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIE
LIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELGYLRLEPKLMGMLEKDSPEQR
LFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLA
KNIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRL
YFGQPLQELRIDQLALLVGMVKGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQ
AVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKADSGLRVFTSLDPVSQSKLEQA
IHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKP
AVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVP
TVALGMKLGIDQVSATLGKLGVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAP
LSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQGVQEGTGRYLNAQFSSAALA
GKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQ
LPWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW