Protein Info for CSW01_02970 in Vibrio cholerae E7946 ATCC 55056

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 transmembrane" amino acids 191 to 204 (14 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 7 to 515 (509 residues), 721.6 bits, see alignment E=2.4e-221 PF04262: Glu_cys_ligase" amino acids 13 to 378 (366 residues), 560.8 bits, see alignment E=5.4e-173

Best Hits

Swiss-Prot: 100% identical to GSH1_VIBCH: Glutamate--cysteine ligase (gshA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to vch:VC0556)

MetaCyc: 59% identical to glutamate--cysteine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>CSW01_02970 glutamate--cysteine ligase (Vibrio cholerae E7946 ATCC 55056)
MSKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDF
AESLLEFITPVSHEVDTLLAQMSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGS
SNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDALFGEQEESARQASKSAAYF
GVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYT
NSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAP
IRPKRVTQSGERPSQALARAGVEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDS
DPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTLQAWAKRVFADLRVIAETMDA
ALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQ
FYSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS