Protein Info for CSW01_02475 in Vibrio cholerae E7946 ATCC 55056

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR00234: tyrosine--tRNA ligase" amino acids 11 to 429 (419 residues), 403.2 bits, see alignment E=7.1e-125 PF00579: tRNA-synt_1b" amino acids 41 to 337 (297 residues), 276.1 bits, see alignment E=5.4e-86 PF22421: SYY_C-terminal" amino acids 348 to 427 (80 residues), 61.7 bits, see alignment E=7.5e-21 PF01479: S4" amino acids 368 to 400 (33 residues), 36.9 bits, see alignment (E = 3.5e-13)

Best Hits

Swiss-Prot: 100% identical to SYY1_VIBCH: Tyrosine--tRNA ligase 1 (tyrS1) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 100% identity to vch:VC0465)

MetaCyc: 60% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.1

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>CSW01_02475 tyrosine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MPRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLV
PLLMLKRFQNAGHQAIALIGGATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMT
SQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMINRESVKQRLARPDQGISFTE
FSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSD
GTKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADAL
RAGKPQAQQILAEHVTRFVHGEEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVA
ASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQQEGGLSGFALFDRYYVLQRG
KKQFALIKLEGLAE