Protein Info for CSW01_02430 in Vibrio cholerae E7946 ATCC 55056

Annotation: non-canonical purine NTP pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00042: non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family
  • amino acids 3 to 193 (191 residues), 212.2 bits, E=2.1e-67
• PF01725: Ham1p_like
  • amino acids 4 to 192 (189 residues), 230.1 bits, E=9.7e-73

Best Hits

Swiss-Prot: 100% identical to IXTPA_VIBCH: dITP/XTP pyrophosphatase (VC_0456) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 100% identity to vch:VC0456)

MetaCyc: 66% identical to dITP/XTP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]

Predicted SEED Role

"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (7/9 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• dZTP biosynthesis (3/5 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Purine metabolism
• Riboflavin metabolism
• Starch and sucrose metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.66 or 3.6.1.9

Protein Sequence (200 amino acids)

>CSW01_02430 non-canonical purine NTP pyrophosphatase (Vibrio cholerae E7946 ATCC 55056)
MKKIVLATGNQGKVREMADLLSDFGFDVVAQSEFNVPEAAETGTTFIENAIIKARHAAQI
TGLPTIADDSGLEVDYLNGAPGIYSARYAGEHASDGDNLNKLLMAMQDVPDDQRSARFHC
VLVLMRHADDPTPIVCHGKWEGKILTAPHGSNGFGYDPIFWVPEENCASAELEPVRKKQL
SHRGKALQKLFKAIEEQRTC