Protein Info for CSW01_01705 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA-directed RNA polymerase subunit beta'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1401 PF04997: RNA_pol_Rpb1_1" amino acids 15 to 342 (328 residues), 288.8 bits, see alignment E=1.5e-89 TIGR02386: DNA-directed RNA polymerase, beta' subunit" amino acids 19 to 1366 (1348 residues), 1789.5 bits, see alignment E=0 PF00623: RNA_pol_Rpb1_2" amino acids 344 to 395 (52 residues), 33.9 bits, see alignment (E = 9.6e-12) amino acids 389 to 485 (97 residues), 97.7 bits, see alignment 2.3e-31 PF04983: RNA_pol_Rpb1_3" amino acids 489 to 644 (156 residues), 110.7 bits, see alignment E=1.7e-35 PF05000: RNA_pol_Rpb1_4" amino acids 674 to 763 (90 residues), 76 bits, see alignment 5e-25 PF04998: RNA_pol_Rpb1_5" amino acids 766 to 1314 (549 residues), 262.7 bits, see alignment E=1e-81

Best Hits

Swiss-Prot: 82% identical to RPOC_ACTP2: DNA-directed RNA polymerase subunit beta' (rpoC) from Actinobacillus pleuropneumoniae serotype 5b (strain L20)

KEGG orthology group: K03046, DNA-directed RNA polymerase subunit beta' [EC: 2.7.7.6] (inferred from 82% identity to aat:D11S_0064)

MetaCyc: 82% identical to RNA polymerase subunit beta' (Escherichia coli K-12 substr. MG1655)
DNA-directed RNA polymerase. [EC: 2.7.7.6]

Predicted SEED Role

"DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1401 amino acids)

>CSW01_01705 DNA-directed RNA polymerase subunit beta' (Vibrio cholerae E7946 ATCC 55056)
MKDLLNFLKAQHKTEEFDAIKIGLASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRDRMGHIELASPVAHIWFLKSL
PSRIGLLMDMPLRDIERVLYFEMYVVTEPGMTDLERGQMLTEEEYLDRLEEWGDEFTAKM
GAEAIKDLLASMDLPAEAEQMREELDTTNSETKRKKLTKRLKLVEAFVASGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLELAAPDIIVRNEKRMLQ
ESVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYSKLETRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RTLMMSTNNILSPASGDPIIVPSQDVVLGLYYMTREKINAKGEGMYLTGPAEAEKAYRTK
TAELHARVKVRITETIKHENGKLTTETKMIDTTVGRAMLWQIVPKGLPYSLVNQKLGKKQ
ISNLLNEAYRKLGLKDTVIFADQIMYTGFAYAALSGVSVGIDDMVVPAAKYTEIAEAEEE
VREIQEQFQSGLVTAGERYNKVIDIWASTNDRVAKAMMENLSSEQVINRQGEQEKQESFN
SIYMMADSGARGSAAQIRQLAGMRGLMARPDGSIIETPITANFKEGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDVVVTEHDCGTLEGVVMTPHIEGGDVKVALTELAL
GRVVSEDILKPGTDEVLIPRNTLLDEKWCKVINDNSVDQIKVRSVVTCDSDFGCCAQCYG
RDLARGHLVNQGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASTAAAENSIQAKNNGSVKL
HNAKFVTNKDGKLVITSRASELTIIDEFGRTKEKHKLPYGSMLSKADGDAVAAGETVANW
EAHTMPIITEVAGRVQFVDMIDGVTVSRQTDDLTGLSSSEVTEAAARPAAGKDMRPAIKL
VDANGKDVLIPGTDMPAQYFLPGKAIVNLDDGAEVNVGDTLARIPQKSGGNKDITGGLPR
VADLFEARKPKEPAILAEHSGTVSFGKETKGKRRLIITRDSGDTYEEMIPKHRQLNVFEG
ERIERGDVIADGPESPHDILRLRGIHAVTTYIANEVQEVYRLQGVKINDKHIETIVRQML
RKCTITFAGDSEFLPGETVEYSQVKIANRKLVEEGKEPARFERELLGITKASLATESFIS
AASFQETTRVLTEAAVSGKRDDLRGLKENVIVGRLIPAGTGFAYHQDRQAKRAQEQQGPS
AEQATDNLAALLNAGFSSDDE