Protein Info for CSW01_01555 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent RNA helicase RhlB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 PF00270: DEAD" amino acids 33 to 207 (175 residues), 153.9 bits, see alignment E=3.4e-49 PF00271: Helicase_C" amino acids 243 to 351 (109 residues), 110.5 bits, see alignment E=5.2e-36

Best Hits

Swiss-Prot: 100% identical to RHLB_VIBCH: ATP-dependent RNA helicase RhlB (rhlB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03732, ATP-dependent RNA helicase RhlB [EC: 3.6.4.13] (inferred from 100% identity to vco:VC0395_A2697)

MetaCyc: 76% identical to ATP-dependent RNA helicase RhlB (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase RhlB" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (438 amino acids)

>CSW01_01555 ATP-dependent RNA helicase RhlB (Vibrio cholerae E7946 ATCC 55056)
MKKTHITEHKFADFGLQPQVIDGLEKKGFVYCTPIQALALPVLLSGQDIAGQAQTGTGKT
LAFLTATFNHLLTTPAAEGRAETQPRAIIMAPTRELAIQIFNDAEPLLASTGLKAALAYG
GESYDKQLAKLQSGVDILIGTTGRIIDFYKQRVFNLNHIQAVVLDEADRMFDLGFIKDIR
FLFRRMPEPKDRLNMLFSATLSYRVQELAFEHMNNPEHVVVEPEQKTGHRIQEELFYPSN
EHKMALLQTLIEEEWPDRAIIFANTKHRCEQIWAHLAADNHRVGLLTGDVPQKKRERILE
QFTQGDVDILVATDVAARGLHIPQVTHVFNYDLPDDCEDYVHRIGRTGRAGASGHSISFA
CEEYAINLPAIESYIEHAIPTSDYDPSALLTDLPAPLSLRSSPQQRRTNTAGSRNSNNGG
NRKPQQRRPRAPRPKKEA