Protein Info for CSW01_01045 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 611 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 18 to 606 (589 residues), 856.4 bits, see alignment E=1.1e-261 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 19 to 466 (448 residues), 633.9 bits, see alignment E=1.7e-194 PF00270: DEAD" amino acids 32 to 190 (159 residues), 90.4 bits, see alignment E=2.9e-29 PF00271: Helicase_C" amino acids 228 to 334 (107 residues), 81.8 bits, see alignment E=1.1e-26 PF16124: RecQ_Zn_bind" amino acids 345 to 407 (63 residues), 69.3 bits, see alignment E=9.8e-23 PF09382: RQC" amino acids 409 to 518 (110 residues), 133.5 bits, see alignment E=7.7e-43 PF00570: HRDC" amino acids 537 to 603 (67 residues), 79.7 bits, see alignment E=3.1e-26

Best Hits

Swiss-Prot: 66% identical to RECQ_ECOLI: ATP-dependent DNA helicase RecQ (recQ) from Escherichia coli (strain K12)

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to vcj:VCD_001419)

MetaCyc: 66% identical to ATP-dependent DNA helicase RecQ (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (611 amino acids)

>CSW01_01045 DNA helicase RecQ (Vibrio cholerae E7946 ATCC 55056)
MTATLMAESSALFATPERVLHEVFGYQQFRVGQQEVIEAALAGRDSLVIMPTGGGKSLCY
QIPALVLEGVTLVISPLISLMKDQVDQLKANGVAAECVNSTLAREELIAIYNRMHAGQLK
LLYVSPERVLTAEFIERLSHLPLAMIAVDEAHCISQWGHDFRPEYASLGQLKQRFPNVPV
MALTATADDATRHDIMQRLQLNEPHQYLGSFDRPNIRYMLVEKHKPVSQVIRYLETQRGQ
CGIIYCGSRKKVEMLTEKLCGNHIRAASYHAGMDADERAWVQEAFQRDDLQIVVATVAFG
MGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMMLYDPADMNWLRRMLDEKPD
GAQKQVESHKLTAMGAFAEAQTCRRQVLLNYFGEYRDKPCGNCDICLDPPKRFDATEEAR
KALSCVYRVNQNFGIGYVVEVLRGMQNIRIREHGHDKITTYGIGRDHSHDYWVSIFRQLI
HKGMLYQNITRNSTLQLTEEARPLLRGEMNIELAVPRLDTAARAAKSDKLSGKNYDKKLF
AKLRKLRKSIADDEGLPPYVVFSDATLIDMAEIMPTSYGEMLAVNGVGQRKLEKYADPFL
DLIQEHLTYHG