Protein Info for CSW01_01005 in Vibrio cholerae E7946 ATCC 55056

Annotation: 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 279 PF04378: RsmJ" amino acids 33 to 277 (245 residues), 415.5 bits, see alignment E=3.1e-129

Best Hits

Swiss-Prot: 64% identical to RLMJ_ECOLI: Ribosomal RNA large subunit methyltransferase J (rlmJ) from Escherichia coli (strain K12)

KEGG orthology group: K07115, (no description) (inferred from 100% identity to vco:VC0395_A2569)

MetaCyc: 64% identical to 23S rRNA m6A2030 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6998 [EC: 2.1.1.266]

Predicted SEED Role

"Protein involved in catabolism of external DNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.266

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (279 amino acids)

>CSW01_01005 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ (Vibrio cholerae E7946 ATCC 55056)
MLSYRHSFHAGNHADVLKHIVQSLILNSLQQKEKPFVYHDTHSGVGRYDLTHEWSEKTGE
YKQGIARIWQQDNIPAELDSYLDAIRQLNQGETLRYYPGSPRVARAHLREQDRMVLTELH
PSDYPLLEQEFHRDRQVSIYKEDGFARLKASLPPQERRGLVLIDPPYELAKEYRDVVRAI
AQSYKRWATGIYAIWYPVVNRCDIDDMLEGLQGLEIRKILQIELGVAPDTNERGMTASGM
IVINPPWTLESQMQTILPFLKQAIAPATGHYKVEWVVPE