Protein Info for CSW01_00610 in Vibrio cholerae E7946 ATCC 55056

Annotation: hydroxymethylbilane synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00212: hydroxymethylbilane synthase
  • amino acids 12 to 303 (292 residues), 377.4 bits, E=1.9e-117
• PF01379: Porphobil_deam
  • amino acids 12 to 219 (208 residues), 292.2 bits, E=2e-91
• PF03900: Porphobil_deamC
  • amino acids 233 to 301 (69 residues), 74.8 bits, E=5.9e-25

Best Hits

Swiss-Prot: 100% identical to HEM3_VIBCH: Porphobilinogen deaminase (hemC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01749, hydroxymethylbilane synthase [EC: 2.5.1.61] (inferred from 100% identity to vcj:VCD_001580)

MetaCyc: 76% identical to hydroxymethylbilane synthase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylbilane synthase. [EC: 2.5.1.61]

Predicted SEED Role

"Porphobilinogen deaminase (EC 2.5.1.61)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 2.5.1.61)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• superpathway of heme b biosynthesis from glycine (7/8 steps found)
• dipyrromethane cofactor biosynthesis (3/4 steps found)
• tetrapyrrole biosynthesis II (from glycine) (3/4 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (18/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (18/36 steps found)
• superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.61

Protein Sequence (317 amino acids)

>CSW01_00610 hydroxymethylbilane synthase (Vibrio cholerae E7946 ATCC 55056)
MDRNIIMTETPIRIATRQSPLALWQANYVKDALMAAHPGLQVELVTMVTRGDVILDTPLA
KVGGKGLFVKELEIAMLEGRADLAVHSMKDVPVDFPDGLGLVTICEREDPRDAFVSNTYA
KIEDLPSGAIVGTCSLRRQCQLKAARPDLVIKELRGNVGTRLSKLDAGEYDAIILAAAGL
KRLELESRIRSFIEPEQSLPAVGQGAVGIECRVNDQRVRALLAPLNHADTADRVRCERAM
NLTLQGGCQVPIGSYALLEGDTIWLRALVGEPDGSQIVRGEIRGPRTQAEQLGITLAEQL
LSQGAKEILERLYCDHE