Protein Info for CSW01_00560 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 PF02739: 5_3_exonuc_N" amino acids 9 to 170 (162 residues), 195 bits, see alignment E=1.9e-61 TIGR00593: DNA polymerase I" amino acids 9 to 934 (926 residues), 944.9 bits, see alignment E=3e-288 PF01367: 5_3_exonuc" amino acids 171 to 272 (102 residues), 107.9 bits, see alignment E=7.4e-35 PF01612: DNA_pol_A_exo1" amino acids 335 to 521 (187 residues), 123.8 bits, see alignment E=1.7e-39 PF22619: DNA_polI_exo1" amino acids 395 to 489 (95 residues), 43.2 bits, see alignment E=8.6e-15 PF00476: DNA_pol_A" amino acids 557 to 931 (375 residues), 554 bits, see alignment E=4.3e-170

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 62% identity to amc:MADE_00016)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>CSW01_00560 DNA polymerase I (Vibrio cholerae E7946 ATCC 55056)
MAHIPDNPLILIDGSSYLYRAFHAYPGTMSNGEIPTNAIYGVVNMIRSMMRQFASDRMAV
IFDAKGKTFRDEMYDQYKAHRPPMPDELRCQVEPLHQVIRAMGLPLLAIEGVEADDVIGT
LARQASQAGMPVLISTGDKDMAQLVDDNITLINTMTNVVLDREGVIEKFGIPPELIIDYL
ALMGDKVDNIPGVPGVGEKTATALLQGIGGLEALYANLDKIAALGFRGSKTMAQKLEENR
GNAKLSYQLATIKCDVELEESPQTLLKQTPDRDALMSLYGKLAFKSWLTELLDGGTGIVT
ADEQTKTSSVTVSTAATHAAAIPESPAAHIDRSQYQTILNEQDFQLWLEKLKQAELFAFD
TETDNLDYMVANLVGMSFAVAEGEAAYLPVAHDYLDAPQQLERDWVIAQLKPLLEDESKA
KVGQNLKYDASVMARYGVELRGIRHDTMLQSYVYNSVGGKHDMDSLALRFLQHSCISFEQ
VAGKGKNQLTFNQIALEEAAQYAAEDADVTLRLHQRIHPLIEQDAKLEQVYREIEMPLVP
VLSRIERTGVMIDDMLLSAQSQEIALRLDQLEQNAYELAGQPFNLSSPKQLQTILFEQMK
LPVLQKTPSGTPSTNEEVLQELALDYPLPKVLIEYRGLAKLKSTYTDKLPKMINPSTGRV
HTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFVAPHGWKIMAVDYSQIELRIMA
HLSGDQALLDAFRDGKDIHAATAAEIIGVPIDQVSSEQRRRAKAVNFGLIYGMSAFGLAK
QLGIPRGEAQEYMDKYFERYPGVMQYMEDTRSRAAQLGYVETIFGRRLHLPEITSRNAMR
RKAAERAAINAPMQGTAADIIKKAMLLVDEWIEREGDGRVKLLMQVHDELVFEVKESSLS
EIESKVQQLMESAAELAVPLVAEAGHGDNWEQAH