Protein Info for CSW01_00260 in Vibrio cholerae E7946 ATCC 55056

Annotation: 5-(carboxyamino)imidazole ribonucleotide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01161: phosphoribosylaminoimidazole carboxylase, ATPase subunit" amino acids 3 to 350 (348 residues), 349.9 bits, see alignment E=9.4e-109 PF02786: CPSase_L_D2" amino acids 92 to 266 (175 residues), 23.3 bits, see alignment E=6.3e-09 PF02222: ATP-grasp" amino acids 101 to 274 (174 residues), 173.3 bits, see alignment E=5.3e-55 PF17769: PurK_C" amino acids 300 to 351 (52 residues), 70.3 bits, see alignment 1.3e-23

Best Hits

Swiss-Prot: 100% identical to PURK_VIBCH: N5-carboxyaminoimidazole ribonucleotide synthase (purK) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 100% identity to vch:VC0051)

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21

Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>CSW01_00260 5-(carboxyamino)imidazole ribonucleotide synthase (Vibrio cholerae E7946 ATCC 55056)
MRVLVLGAGQLARMMSLAGAPLNIETIAFDVGSENIVHPLTQTVLGHGLEQAIEQVDVIT
AEFEHIPHPILDLCARSGKLYPSAEAIKAGGDRRLEKALLDRAQVANARYTMIRSRDDLT
SAIAEIGLPMVLKSALGGYDGKGQWRLKEPTQIESVWQELAQYLAANPEQAIVAEEFVAF
DREVSLVGARNLVGDVVVYPLAENVHTQGVLSLSTAIDAPALQTQAKAMFKAVAEQLNYV
GVLALEFFEVQGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRET
AMINILGEDQLPAEVLALEGCHVHWYGKAKRSGRKMGHINVTADYSGELQRKLCQLATVL
DEKAFPAVHAVAKEIQP