Protein Info for CCNA_03242 in Caulobacter crescentus NA1000

Annotation: succinic semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 PF00171: Aldedh" amino acids 20 to 477 (458 residues), 573.5 bits, see alignment E=1.4e-176 TIGR01780: succinate-semialdehyde dehydrogenase" amino acids 29 to 476 (448 residues), 683.4 bits, see alignment E=7.2e-210

Best Hits

Swiss-Prot: 61% identical to GABD_ECOLI: Succinate-semialdehyde dehydrogenase [NADP(+)] GabD (gabD) from Escherichia coli (strain K12)

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 100% identity to ccs:CCNA_03242)

MetaCyc: 61% identical to succinate-semialdehyde dehydrogenase (NADP+) GabD (Escherichia coli K-12 substr. MG1655)
SUCCSEMIALDDEHYDROG-RXN [EC: 1.2.1.79]; Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.79, 1.2.1.20]

Predicted SEED Role

"Aldehyde dehydrogenase (EC 1.2.1.3)" in subsystem Entner-Doudoroff Pathway or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.3

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20 or 1.2.1.3 or 1.2.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3CEE2 at UniProt or InterPro

Protein Sequence (485 amino acids)

>CCNA_03242 succinic semialdehyde dehydrogenase (Caulobacter crescentus NA1000)
MTCMTALNLVETAALIDGQWVRGEASFDVLNPADGTLIAAVADLGAAETTLAIDAAHRAL
PAWAARTAKERGAILRRWSDLILAHADDLARLMTDEQGKPLAEAKGEVVYGASFIDWFAE
EAKRAYGHTIPTPMPGKRLASIKQPVGVCAAIAPWNFPIAMITRKVGPALAAGCTVVVKP
AAETPLSALAIARLATEAGVPAGVLNIVTTTRSSEVGKVLCDDSRVRKLSFTGSTPIGKV
LYQQCAGTMKKLSLELGGNAPFIVFDDADLEAAVDGAIASKYRNAGQTCVCANRLIVQSG
IHDAFAARLAEKVAALKVGPGTGEGVQIGPLINEKALTKVVGLVSGAVQAGAEVLTGGDV
HGLGGHFYQPTVLVGATPEMRIFQEEIFGPVAPIVKFETEAEAVELANATPFGLAAYFYS
RDVGRCWRVAEQIEAGMVGINEGLISTEVAPFGGVKESGLGREGASEGLDEYLETKYLCF
GGVAG