Protein Info for CCNA_02549 in Caulobacter crescentus NA1000

Annotation: glucokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00480: ROK
  • amino acids 4 to 293 (290 residues), 250.6 bits, E=1.2e-78

Best Hits

Swiss-Prot: 54% identical to MAK_ECOLI: Fructokinase (mak) from Escherichia coli (strain K12)

KEGG orthology group: K00847, fructokinase [EC: 2.7.1.4] (inferred from 100% identity to ccs:CCNA_02549)

MetaCyc: 54% identical to fructokinase (Escherichia coli K-12 substr. MG1655)
Mannokinase. [EC: 2.7.1.7]; Fructokinase. [EC: 2.7.1.7, 2.7.1.4]

Predicted SEED Role

"ROK family Glucokinase with ambiguous substrate specificity"

MetaCyc Pathways

• sucrose degradation IV (sucrose phosphorylase) (4/4 steps found)
• D-mannose degradation II (2/2 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• mannitol degradation II (3/4 steps found)
• sucrose degradation III (sucrose invertase) (3/4 steps found)
• D-sorbitol degradation I (2/3 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• sucrose degradation VII (sucrose 3-dehydrogenase) (2/4 steps found)
• heterolactic fermentation (12/18 steps found)
• sucrose degradation I (sucrose phosphotransferase) (1/3 steps found)
• mannitol cycle (2/5 steps found)
• CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis (1/4 steps found)
• superpathway of CMP-sialic acids biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.4

Use Curated BLAST to search for 2.7.1.4 or 2.7.1.7

Sequence Analysis Tools

See A0A0H3CAT2 at UniProt or InterPro

Protein Sequence (296 amino acids)

>CCNA_02549 glucokinase (Caulobacter crescentus NA1000)
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCAR
LGLGIPGSISPRTGLIRNANSTYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAG
AGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAPLPWPKPEEYPGPDCWCGRKG
CLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV