Protein Info for CCNA_02484 in Caulobacter crescentus NA1000

Annotation: trans-feruloyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 13 to 259 (247 residues), 160.6 bits, E=4.7e-51
• PF16113: ECH_2
  • amino acids 17 to 202 (186 residues), 102.4 bits, E=3.7e-33

Best Hits

Swiss-Prot: 54% identical to HCHL_PSEFL: Hydroxycinnamoyl-CoA hydratase-lyase from Pseudomonas fluorescens

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to ccs:CCNA_02484)

MetaCyc: 71% identical to feruloyl-CoA hydratase/lyase (Sphingomonas sp. SYK6)
4.1.2.41-RXN [EC: 4.1.2.61]; 4.1.2.61 [EC: 4.1.2.61]; 4.1.2.61 [EC: 4.1.2.61]; 4.1.2.61 [EC: 4.1.2.61]; 4.1.2.61 [EC: 4.1.2.61]; 4.1.2.61 [EC: 4.1.2.61]

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Butanol Biosynthesis or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 4.2.1.17)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• fatty acid salvage (6/6 steps found)
• adipate degradation (5/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• ferulate degradation (3/3 steps found)
• 4-coumarate degradation (aerobic) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
• trans-caffeate degradation (aerobic) (3/4 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• 4-coumarate degradation (anaerobic) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• valproate β-oxidation (5/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• capsaicin biosynthesis (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• superpathway of phenylethylamine degradation (5/11 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (3/9 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• gallate degradation III (anaerobic) (1/11 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (4/17 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.1.2.61 or 4.2.1.17

Sequence Analysis Tools

See A0A0H3C972 at UniProt or InterPro

Protein Sequence (275 amino acids)

>CCNA_02484 trans-feruloyl-CoA hydratase (Caulobacter crescentus NA1000)
MTDANDTATYRVENRIAWVKFNRPDKRNCMSPTLNRRMMEVLDELEFRDDVGVLVLTGEG
SAWSAGMDLKEYFRETEAKGLGATRQAQRESYGWWRRLRWYQKPTIAMVNGWCFGGGYGP
LYSCDLAIAADEAQFALSEINWGILPGGGATKVVVDLMPLRDAMYHAMTGELIDGRKAAA
WRLVNESVPLERLEARVRELAEVLLKKNPVALKATKDAVRRVKEMNYDNAEDYLVRAQEA
ANSFDNDGRKEGIKQFIDDKTYKPGLGAYDLAKQS