Protein Info for CCNA_02224 in Caulobacter crescentus NA1000

Annotation: methylenetetrahydrofolate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02219: MTHFR
  • amino acids 20 to 301 (282 residues), 309.6 bits, E=1.1e-96
• TIGR00676: methylenetetrahydrofolate reductase [NAD(P)H]
  • amino acids 25 to 300 (276 residues), 334.4 bits, E=2.6e-104

Best Hits

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 100% identity to ccr:CC_2140)

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

• folate transformations I (8/13 steps found)

KEGG Metabolic Maps

• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

See A0A0H3C9A8 at UniProt or InterPro

Protein Sequence (314 amino acids)

>CCNA_02224 methylenetetrahydrofolate reductase (Caulobacter crescentus NA1000)
MTLPPTRRVIGPVARAGERTGRPRVSFEFFPPKTPQMEESLWQAITRLAPLDPAFVSVTY
GAGGSTRERTHRTVKRILDETSLKPAAHLTCVGASREEVDEVIREYWETGVRHIVSLRGD
PPPGEGGIGGVYVPRADGYANATELTKAVRAIAPFEVLVGVYPEKHPESPSLEHDIDVLK
QKVDAGATLGISQFFFDLDAFLRFVDKVRAAGITIPIVPGIMPVTNFAGLKKMAAACQTA
IPSWLGNLFDGLENDAETRRLIACSVAAEMCAKLQEQGFEDFHFYTLNRADLVYAICRVL
GVREISPAASEVAA