Protein Info for CCNA_00931 in Caulobacter crescentus NA1000

Annotation: GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 2 to 197 (196 residues), 237.6 bits, see alignment E=9.5e-75 PF00926: DHBP_synthase" amino acids 6 to 195 (190 residues), 276.5 bits, see alignment E=9.9e-87 TIGR00505: GTP cyclohydrolase II" amino acids 207 to 396 (190 residues), 214.1 bits, see alignment E=1.2e-67 PF00925: GTP_cyclohydro2" amino acids 208 to 372 (165 residues), 213.5 bits, see alignment E=1.4e-67

Best Hits

Swiss-Prot: 55% identical to RIBBA_AQUAE: Riboflavin biosynthesis protein RibBA (ribBA) from Aquifex aeolicus (strain VF5)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to ccr:CC_0887)

MetaCyc: 44% identical to GTP cyclohydrolase II (Chlamydia trachomatis)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C6U2 at UniProt or InterPro

Protein Sequence (400 amino acids)

>CCNA_00931 GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase (Caulobacter crescentus NA1000)
MSRLSEALTRLKAGGMIVLVDDEDRENEGDLVMAAEFADAAAIAFMAKRASGLICLTLEA
ETIDRLCLAPMVSDNRTSRQTAFTVSIEAATGVDTGISAFDRARTIAAAIAPDAQAADLV
SPGHVFPLRARPGGVLVRNGHTEASVDLMRMAGLRPAGVICEIMKDDGSMARRPDLEVFC
REHDLPLLTIAELVAHRKATELLVDEVAAARLPSDHAGQAFSVRAFRSRIDGGEHLAMVA
GPLTGAPLVRVHSECLTGDALGSRRCDCGEQLRESLRQISDSGNGALIYLRNHEGRGLGL
ANKIAAYALQDQGLDTVQANHALGFADDQRDYGVAAQILRALGVGEVKLLSNNPRKAAGL
RANGVTVRREVPLLIPANPDNAAYLETKRDKLGHSLARAS