Protein Info for CCNA_00250 in Caulobacter crescentus NA1000

Annotation: poly(3-hydroxyalkanoate) depolymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 100% identity to ccs:CCNA_00250)

Predicted SEED Role

"putative intracellular PHB depolymerase"

KEGG Metabolic Maps

Butanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H3C4T8 at UniProt or InterPro

Protein Sequence (420 amino acids)

>CCNA_00250 poly(3-hydroxyalkanoate) depolymerase (Caulobacter crescentus NA1000)
MLYALHEAAYYASTPMRLAALAARDFWGSPLNPAADSAVGRRMHAGADLFANVTRRYGKP
KWNIDKVKVGQVDVRVRPTVVWESPWARLVQFDRDMADMRRAGKFSLDPAVLIVAPLSGH
YATLLRGTVEAFLPDHAVFIVDWVNAREVSVLEGRFDFHDYIDHVIQMLEVLGPRPNVVA
VCQPGPPVLAAAALMAEQNHPSRPASMTFMGSPIDARLSPTVTNQLAEEKPFTWFQSNMI
YNVPPPYAGAGRRVYPGFVQLASFMSMNADRHQEAHRRYFNHLVSGDGDSADKHLEFYDE
YLSVLDLTEEFYLQTIDIVFQQYLLPKGELVHRGTRVKPEVITDIGLMTVEGENDDISGI
GQTQAAHGLCANIPEDMKLDYVQPSVGHYGVFNGRRFREEIYPRVRKFIRKCDPNFGEEV