Protein Info for CCNA_00033 in Caulobacter crescentus NA1000

Annotation: polyribonucleotide nucleotidyltransferase/polynucleotide adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 10 to 693 (684 residues), 1056.6 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 144 (131 residues), 110.4 bits, see alignment E=2.3e-35 amino acids 326 to 458 (133 residues), 96.9 bits, see alignment E=3.2e-31 PF03725: RNase_PH_C" amino acids 147 to 210 (64 residues), 49.2 bits, see alignment E=1e-16 amino acids 462 to 531 (70 residues), 29.5 bits, see alignment E=1.5e-10 PF03726: PNPase" amino acids 241 to 322 (82 residues), 66.1 bits, see alignment E=7.9e-22 PF00013: KH_1" amino acids 558 to 615 (58 residues), 40.8 bits, see alignment 3.7e-14 PF00575: S1" amino acids 621 to 692 (72 residues), 65 bits, see alignment E=1.7e-21

Best Hits

Swiss-Prot: 100% identical to PNP_CAUVC: Polyribonucleotide nucleotidyltransferase (pnp) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to ccs:CCNA_00033)

MetaCyc: 57% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B8GWZ0 at UniProt or InterPro

Protein Sequence (712 amino acids)

>CCNA_00033 polyribonucleotide nucleotidyltransferase/polynucleotide adenylyltransferase (Caulobacter crescentus NA1000)
MFDIKRKTIEWGGKTLVLETGRIARQADGAVLATMGETVVLATAVFAKSQKPGQDFFPLT
VNYQEKTFAAGKIPGGFFKREGRPSEKETLVSRLIDRPIRPLFVKGFKNEVQVVVTVLQH
DLENDPDILGMVAASAALCLSGAPFMGPIGAARVGWVDGAYVLNPTLDEMKESKMDLVVA
GTADAVMMVESEIQELSEEIVLGGVNFAHQQMQAVIDAIIDLAEHAAKEPFAFEPEDTDA
IKAKMKDLVGADIAAAYKIQKKQDRYEAVGAAKKKAIAALGLSDENPTGYDPLKLGAIFK
ELEADVVRRGILDTGLRIDGRDVKTVRPILGEVGILPRTHGSALFTRGETQAIVVATLGT
GDDEQFIDALEGTYKESFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRALRPMLPTK
EDFPYTIRLVSEITESNGSSSMATVCGSSLAMMDAGVPLVRPVSGIAMGLILEQDGFAVL
SDILGDEDHLGDMDFKVAGTSEGLTSLQMDIKIAGITPAIMEQALAQAKEGRAHILGEMN
KAMDAPRADVGDFAPKIETINIPTDKIREVIGSGGKVIREIVATTGAKVDINDDGVVKVS
ASDGAKIKAAIDWIKSITDEAEVGKIYDGKVVKVVDFGAFVNFFGAKDGLVHVSQISNER
VAKPSDVLKEGQMVKVKLLGFDDRGKTKLSMKVVDQETGEDLSKKEAAAEEA