Protein Info for CA265_RS24655 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): maltose phosphorylase (EC 2.4.1.8)
Rationale: Specifically important for utilizing D-maltose.
Original annotation: family 65 glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 772 PF03636: Glyco_hydro_65N" amino acids 14 to 265 (252 residues), 253.9 bits, see alignment E=2.5e-79 PF03632: Glyco_hydro_65m" amino acids 322 to 688 (367 residues), 468.8 bits, see alignment E=1.8e-144 PF03633: Glyco_hydro_65C" amino acids 697 to 758 (62 residues), 56.7 bits, see alignment 2.3e-19

Best Hits

KEGG orthology group: None (inferred from 70% identity to cpi:Cpin_5087)

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.64 or 2.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZBM0 at UniProt or InterPro

Protein Sequence (772 amino acids)

>CA265_RS24655 maltose phosphorylase (EC 2.4.1.8) (Pedobacter sp. GW460-11-11-14-LB5)
MKNYIKADEWNIIEEGFDPHLNKISESIFSLGNGRMGQRANFEETYTGETLPGNYVAGVY
YPDKTRVGWWKNGYPEYFAKVLNAANWIGIEVKLDGEILDLATAEVSDFKRVLNMHAGYL
ERTFTAKLKSGKTLKVKSTRFCSIADDEVGAIRYSITPLNFDGRLTLMPFIDGDVKNQDS
NYDEKFWDKVADEISGTEAYIKLRTKKTEFEVCTGSNIELYKNAEKLAINPEAVRKEKFV
GQTFSLEVKANEEISLVKIAANLSSENYPKESLLKETKSIIAKASAKGFDTLLKEQTEAW
ASKWEESDIIIEGDVSAQQAIRFNIFQLFQTYTGKDDRLNIGPKGFTGEKYGGSTYWDTE
AYCVPFYLATAPQEVSKNLLVYRHKQLGKAIENAAKLGFKDGAALYPMVTMNGEECHNEW
EITFEEIHRNGAIAFAIFNYIRYTGDESYLSDFGLEVLIGIARFWKQRVNWSNDKQQYVM
LGVTGPNEYENNVNNNWYTNILATWCMKYATEAAEIVKTQQPEKYNSLLKSLNFDQKEFA
DWADIIEKMYYPQDEKLGIFLQQDGYLDKEQTLVKDLPASERPINQKWSWDRILRSCFIK
QADVLQGLYFFEEDYDLDTLKRNFDFYEPRTVHESSLSPCVHSILAAKLNDEARAYEFYL
RTARLDLDDYNNDTEDGLHITSMAGTWMSVVEGFAGMRVREGKLQFNPFLPGKWKSFSFT
IGFRGATLKINITESGISIKNNAAVDLEIGIRNQLYKLAGNTEIEVNNAELV