Protein Info for CA265_RS19875 in Pedobacter sp. GW460-11-11-14-LB5
Updated annotation (from data): Altronate dehydratase (EC 4.2.1.7)
Rationale: Specifically important for utilizing D-Galacturonic Acid monohydrate. Automated validation from mutant phenotype: the predicted function (ALTRODEHYDRAT-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: altronate hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01685, altronate hydrolase [EC: 4.2.1.7] (inferred from 73% identity to phe:Phep_4019)Predicted SEED Role
"Altronate dehydratase (EC 4.2.1.7)" (EC 4.2.1.7)
MetaCyc Pathways
- superpathway of hexuronide and hexuronate degradation (10/10 steps found)
- D-galacturonate degradation I (5/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (18/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.7
Use Curated BLAST to search for 4.2.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9Z9E6 at UniProt or InterPro
Protein Sequence (548 amino acids)
>CA265_RS19875 Altronate dehydratase (EC 4.2.1.7) (Pedobacter sp. GW460-11-11-14-LB5) MVTRILKIHPNDNVLVALQNLAKGETVIYDGHEYILQDDIQAKHKFFMQDMNAGDHIIMY GVLVGKAQHFILKGGLMDTENTKHASDPYEFRPYHYEWHAPDVSKFEGRTFNGYIRSDGR VGTANYWLFIPTVFCENRNLDVIREALHNELGYAVTDKYKSYAHQLVEAYKNGEILAEAD PSSIGLANPSANRVFKNVDGIKFLNHQGGCGGTRQDAAVLSKLLAAYADHPNVAGVTVLS LGCQNLQVKDFMDDLKHRSPNFDKPLFVFEQQQSQSEEQLVKEAIRKTFIGLTEINKIER QPAPLSKLVLGVKCGGSDGFSGISANPAVGYTSDLLVALGGTVLLAEFPELCGAEQQLID RTKDETAARKFIQLMTAYNQSAENVGSGFFMNPSPGNIKDGLITDAIKSTGAAKKGGTSP VEDVLDYTEPATKPGLNLVCTPGNDVEATTGKAASGATLILFTTGLGTPTGNPVCPTIKV STNNALTKRMGDIIDINCGPVIEGEKTIEQMGEDILEYCIKAASGEVIPKAVLLNQDDFI PWKRGVSL