Protein Info for CA265_RS19870 in Pedobacter sp. GW460-11-11-14-LB5
Updated annotation (from data): Glucuronate isomerase (EC 5.3.1.12)
Rationale: Specifically important for utilizing D-Galacturonic Acid monohydrate. Automated validation from mutant phenotype: the predicted function (5.3.1.12) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: glucuronate isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to UXAC_FLAJ1: Uronate isomerase (uxaC) from Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101)
KEGG orthology group: K01812, glucuronate isomerase [EC: 5.3.1.12] (inferred from 75% identity to phe:Phep_1162)MetaCyc: 52% identical to D-glucoronate/D-galacturonate isomerase (Escherichia coli K-12 substr. MG1655)
Glucuronate isomerase. [EC: 5.3.1.12]; 5.3.1.12 [EC: 5.3.1.12]
Predicted SEED Role
"Uronate isomerase (EC 5.3.1.12)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 5.3.1.12)
MetaCyc Pathways
- superpathway of hexuronide and hexuronate degradation (10/10 steps found)
- superpathway of β-D-glucuronosides degradation (7/7 steps found)
- D-galacturonate degradation I (5/5 steps found)
- β-D-glucuronide and D-glucuronate degradation (3/3 steps found)
- pectin degradation II (3/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (18/31 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.3.1.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9Z977 at UniProt or InterPro
Protein Sequence (466 amino acids)
>CA265_RS19870 Glucuronate isomerase (EC 5.3.1.12) (Pedobacter sp. GW460-11-11-14-LB5) MKPFLDENFLLQSKTAEKLYHNFAKSLPIIDYHNHLIPEQIANNTQFANISQVWLAGDHY KWRAMRANGVDEKYITGVGSDYEKFEKWAETVPYTLRNPLYHWTHLELQRYFGITDLLSG KTAQKIFDECSAKLQTPEYSVRGLLAKMNVEAVCTTDDPLDSLNFHQQLAREGANLKMLP AFRPDKAMNSDDIEGLNEYIDKLESVADKTISNFQDYIDALKSRHDYFAANGCSVSDHGL EQIYAEDYTEAEIASIFDKIRSKQHISYEENLKFKSAMLVYFAEWDHEKGWVQQYHLGAL RNNNARMLRQLGPDTGWDSIGDFSQARMLSKFLNRLDNQDKLAKTIIYNLNPADNELIAT MIGNFNDGSVAGKVQFGSAWWFLDQKDGMIKQLNALSNMGLVSRLVGMLTDSRSFLSFPR HEYFRRIVCNLFGEDIENGELPNDLEWVGKIVQDISYFNAKNYFKF