Protein Info for CA265_RS17485 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1133 TIGR00392: isoleucine--tRNA ligase" amino acids 6 to 925 (920 residues), 976.1 bits, see alignment E=8.4e-298 PF00133: tRNA-synt_1" amino acids 17 to 717 (701 residues), 394.2 bits, see alignment E=1.3e-121 PF09334: tRNA-synt_1g" amino acids 49 to 186 (138 residues), 28.4 bits, see alignment E=1.5e-10 amino acids 664 to 723 (60 residues), 23 bits, see alignment 6.4e-09 PF08264: Anticodon_1" amino acids 767 to 919 (153 residues), 117.9 bits, see alignment E=7.1e-38 PF19302: DUF5915" amino acids 937 to 1132 (196 residues), 176.2 bits, see alignment E=1.2e-55

Best Hits

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z7V3 at UniProt or InterPro

Protein Sequence (1133 amino acids)

>CA265_RS17485 isoleucine--tRNA ligase (Pedobacter sp. GW460-11-11-14-LB5)
MYSEFKQLELAKIGQEILDFWKKENIFEKSISSRPKSNPFTFYEGPPSANGMPGIHHVMA
RAIKDIFCRYKTLKGYQVKRKGGWDTHGLPIELAVEKKLGITKEDIGKKISVDEYNAACR
TEVMRYTDVWNDLTEKMGYWVDLENPYITYENEYIETLWWILKQLYDKGFLYKGYTVQPY
SPAAGTGLSSHELNQPGTYKDVSDTTIVAQFKAINETLPDALKIFGTVHFLAWTTTPWTL
PSNTALTVGPKIDYVLVKTFNQYTFEPIQVILAKALVGKQFAGKYFLAESDEDLNNFKAE
DKKIPYTILGEFVGADLVGIKYEQLLPYALPYQNPENAFRVISGDFVTTEDGTGIVHTAP
TFGADDARVAKLATPEVPPMLVLDDKGNAVPLVDLQGKFIASLGGNYGGKYVKNEYYKDG
EAPDKSVDVELAIQLKEENKAFKVEKYVHTYPHCWRTDKPVLYYPLDSWFIKTTAVKNKM
VELNKTINWKPESTGIGRFGNWLENLVDWNLSRSRYWGTPLPIWRTADGTEEKCIGSIAD
LNAEIAKSIEAGFMPAGFELKDMHRPYVDDVILTSSKGEKMTRETDLIDVWFDSGAMPYA
QWHFPFENKEEFENAYAADFIAEGVDQTRGWFFTLHAIAVMLSEASDEIKAVNAKVGNPG
VAFKNVVSNGLVLDKNGNKMSKRLGNAVDPFSTIETYSADATRWYMISNASPWDNLKFSL
EGLDEVRRKFFGTLYNTYAFFALYANIDKFEIDKNNLSKVEDRTELDRWILSLLQNLISE
VDESYNTYEPTKATRAIQTFVDEHLSNWYIRLSRRRFWKGEMTDDKRAAYETLYTCLETL
AQLMSPVAPFFADWLYRNLTVTDAYAEESVHLTLWKEADQSLIDNALNERMVYAQDISSM
VLSLRKKSSINVRQPLEKILIPSLNRDFEQKISKVADYILSETNVKHIEFITDTHGIVKK
KLKPNFKSLGKKVGKDMSIVKEALENMNQDDIQRLELDGYINVFGSNNIQHAIDLNDDVE
VFAEDIPGWQVTNLGNLTVALDVTISEELKQEGISRELVNRIQNLRKELNFEVTDRIKVS
LQNDNLVASAVAKNKDYICAEILADEFELTDTVNNANKIVIDDVELSISVTKI