Protein Info for CA265_RS16340 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: acetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 4 to 626 (623 residues), 953.3 bits, E=2.6e-291
• PF16177: ACAS_N
  • amino acids 9 to 64 (56 residues), 73.9 bits, 1.1e-24
• PF00501: AMP-binding
  • amino acids 66 to 452 (387 residues), 293.8 bits, E=2.7e-91
• PF13193: AMP-binding_C
  • amino acids 514 to 592 (79 residues), 76.1 bits, E=5.1e-25

Best Hits

Swiss-Prot: 58% identical to ACSA_BDEBA: Acetyl-coenzyme A synthetase (acsA) from Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 84% identity to phe:Phep_3517)

MetaCyc: 58% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• chitin deacetylation (1/4 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)
• 2-methylcitrate cycle I (1/5 steps found)
• β-alanine biosynthesis II (1/6 steps found)
• 2-methylcitrate cycle II (1/6 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (1/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See A0A1X9Z7L8 at UniProt or InterPro

Protein Sequence (631 amino acids)

>CA265_RS16340 acetate--CoA ligase (Pedobacter sp. GW460-11-11-14-LB5)
MQITSFKQYEEDYKKSVENPEQFWGEVAQNFQWRKPWFKVLSWNFNEPNIKWFEGAKLNI
TENCLDRHLATNGDKPAIVWEPNNPEEESVTYTYKMLHERVCRFANVLKRNGAKKGDRIC
IYMPMVPELAIAVLACARIGAVHSVIFGGFSAKSIADRINDSKCKVVITADGSYRGNKQI
PLKDVIDDALIGCPTVEKCIVLTHIRTPVSMLKGRDVWWEDEVKHVNDICEAEEMDAEDM
LFILYTSGSTGKPKGVVHTCGGYMVYAGYTFSNVFNYQPGEVYFCTADIGWITGHSYIVY
GPLSQGATSVLFEGIPTYPTPSRFWDIVEKHKVNTLYTAPTAIRSLMSYGDDPLNGKDLS
SIRVLGSVGEPINEEAWHWFDEKIGHGKAPIVDTWWQTETGGIMISPIATVTPTKPSFAT
LPLPGIQPILVDENGNEIEGNGVMGNLCIKFPWPGMLRTTYGDHERCKQTYFSTYDNLYF
TGDGCLRDEDGYYRITGRVDDVLNVSGHRIGTAEVENAINMHAGVVESAVVGYPHDVKGQ
GIYAFVIYPEMHGEAELSKKDILQTVTRVIGAIAKPDKILFVSGLPKTRSGKIMRRILRK
IAEGDTSNLGDTSTLLDPGVVEEIIAAAKKL