Protein Info for CA265_RS16120 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 928 PF00133: tRNA-synt_1" amino acids 11 to 144 (134 residues), 52.9 bits, see alignment E=5.3e-18 PF09334: tRNA-synt_1g" amino acids 38 to 145 (108 residues), 52.9 bits, see alignment E=5.3e-18 TIGR00396: leucine--tRNA ligase" amino acids 198 to 928 (731 residues), 720 bits, see alignment E=2e-220 PF13603: tRNA-synt_1_2" amino acids 277 to 458 (182 residues), 198.8 bits, see alignment E=1.2e-62 PF08264: Anticodon_1" amino acids 778 to 889 (112 residues), 57 bits, see alignment E=4.3e-19

Best Hits

Swiss-Prot: 64% identical to SYL_CYTH3: Leucine--tRNA ligase (leuS) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 64% identity to chu:CHU_1287)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z7I2 at UniProt or InterPro

Protein Sequence (928 amino acids)

>CA265_RS16120 leucine--tRNA ligase (Pedobacter sp. GW460-11-11-14-LB5)
MDYQFKEIEQKWQKFWADHQTFKAESNSEKPKYYVLDMFPYPSGAGLHVGHPLGYIASDI
FSRYKRLKGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTEANIATYRRQLDQIGFSFDW
SREVRTSEPSYYKWTQWIFMQLFNSWYNIENDRAEDITTLIEKFNASGTADVKAVCDEDT
KEFMPSDWATFTDEEKQIELLKYRLTYLRESTVNWCPALGTVLANDEVKDGFSERGGFPV
EQKKMMQWSMRITAYSDRLLQGLDTIDWPEPIKEMQRNWIGKSVGASVKFQVEGNDKQIE
VFTTRVDTIFGVSYLVLAPEHEWVAELTTPEQKQDIANYIALTKKKSELDRMADTKTVSG
AFTGTYVINPVSGERVQLWIADYVLAGYGTGAVMGVPSGDQRDWLFATHFNLPIIQILDG
QKDIDVQADPTKEGKYINSGFINGMEYKEAVSFLNNWLEEEKVGKAKVNFRQRDAIFGRQ
RYWGEPIPVYFKDGLPYLVNEEELPLLLPEIDKYLPTETGEPPLARAEDWVPKDGGHYEL
STMPGWAGSSWYWYRYMDANNNNDFASKEAVEYWKDVDLYIGGSEHATGHLLYSRFWNKF
LKDLGYTKEEEPFKKLINQGMIQGRSNFVYRINDENGKPTNTYVSAGLRKEYKTSALHVD
VNIVENDTLDIAKFKAWREEYATAEFILEGGKYICGVEVEKMSKSKFNVVNPDDLIERYG
ADTLRMYEMFLGPLEQSKPWNTNGIEGVFKFLRKFWRLFHNEDWTFHVNDTVPTKAELKS
LHKIIKKVQDDIERFSFNTSVSSFMIAVNELTDLKCKNRQILEDMVIILSPYAPHICEEL
WVLLGNEAGTLSYTAFPTFKPEYLVEDEFAYPVSINGKMKMNLNLSLTLAQPEVESILLA
DEHFQKFLDGKAPKKIIFVKGKIINVVV