Protein Info for CA265_RS15345 in Pedobacter sp. GW460-11-11-14-LB5
Updated annotation (from data): periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13)
Rationale: Specifically important in carbon source D-Trehalose dihydrate; carbon source D-Salicin; carbon source Beta-Lactose; carbon source D-Cellobiose (all are glucosides). Related to lacA of Caulobacter crescentus which is a component of lactose 3-dehydrogenase.
Original annotation: GMC family oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 90% identity to phe:Phep_2474)MetaCyc: 60% identical to D-glucoside-3-dehydrogenase catalytic subunit (Agrobacterium fabrum)
GLUCOSIDE-3-DEHYDROGENASE-RXN [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]
Predicted SEED Role
"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1
MetaCyc Pathways
- sucrose degradation VII (sucrose 3-dehydrogenase) (2/4 steps found)
- lactose degradation II (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.13
Use Curated BLAST to search for 1.1.2.11 or 1.1.99.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9Z7P6 at UniProt or InterPro
Protein Sequence (567 amino acids)
>CA265_RS15345 periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) (Pedobacter sp. GW460-11-11-14-LB5) MMNLNTNLKAENTYDAIVIGSGISGGWAAKELTEKGLRVLMLERGMNIEHITGYETAMKN PWDFKHAGKLTEEQKRTHPVQKRDYPYQEANEKWWVNDLECPYTEDKRFDWYRGFHVGGK SLMWGRQSYRLSDHNFEDNARDGHGSDWPVRYAELSPWYDYAERFAGISGSKENWPTCPD GQFLPPMDLNIVEKSVKARIEEHYKRERIMMIGRVANLTVPHKGRGNCQYRNLCSRGCPF GAYFSTQSSTLPAAMATKRLTLRPYSIVNHIIYDKDTKKAKGVMVIDAETNKTMEFYAKI VFVNGSTLGSTFVLLNSTSEAHPNGLGNGSGQLGHNLMDHHFRCGASGEAEGFDDKYTYG RRANGIYIPRYQNIGNDKRDYLRGFGYQGGASRANWQGDVAELSFGADLKQKMTTPGKWS MGLGGFGEMLPYYENKVYIDKTKKDKWGQPVLAIDCEYKENEKKMRVDMMNDAAEMLEKA GMKNIKTFDNGCYPGMAIHEMGTARMGNDPKTSVLNKWNQMHEVNNVFVTDGSCMPSIAC QNPSLTFMALTARACDYAVKELKKKNI