Protein Info for CA265_RS14790 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: acyl-ACP desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 45% identical to STAD2_ARATH: Stearoyl-[acyl-carrier-protein] 9-desaturase 2, chloroplastic (S-ACP-DES2) from Arabidopsis thaliana

KEGG orthology group: K03921, acyl-[acyl-carrier-protein] desaturase [EC: 1.14.19.2] (inferred from 91% identity to phe:Phep_1163)

MetaCyc: 47% identical to stearoyl-ACP desaturase (Persea americana)
Acyl-[acyl-carrier-protein] desaturase. [EC: 1.14.19.2]; 1.14.19.2 [EC: 1.14.19.2]

Predicted SEED Role

"Probable acyl-ACP desaturase, Stearoyl-ACP desaturase (EC 1.14.19.2)" (EC 1.14.19.2)

MetaCyc Pathways

superpathway of fatty acid biosynthesis II (plant) (39/43 steps found)
oleate biosynthesis I (plants) (2/3 steps found)
palmitoleate biosynthesis II (plants and bacteria) (1/2 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.19.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z6I5 at UniProt or InterPro

Protein Sequence (326 amino acids)

>CA265_RS14790 acyl-ACP desaturase (Pedobacter sp. GW460-11-11-14-LB5)
MSFFADKRREVMVHIEKYMLEMMDTYLKPIDTNWQPSDFLPDSTSETFYQDIRILRDNAK
DLSYDLVAVLIGDTITEEALPTYESWLAMVQGPSMKEDGGWMKWNRHWTAEENRHGDLLN
KYLYLSGRVDMRQMEISTQYLIADGFDIGTGHDPYRNFIYTSFQEMATNISHRRVASLAK
KDGDTLLSRMCGVIASDEARHAKAYKDFMNRIFEVDPNEAMLAFEDMMRQKIVMPAHFLR
EVGLKIGQTFGHFTDAAQRLGVYTAIDYVEIMQQLIEDWKIEGMRDLNEAGEKARDYIMA
LPSRLLRVAERMKNPTIDYKFSWIAG