Protein Info for CA265_RS14755 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 35 to 427 (393 residues), 379.4 bits, E=1.4e-117
• PF08245: Mur_ligase_M
  • amino acids 51 to 270 (220 residues), 55.1 bits, E=5.2e-19

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 59% identity to cpi:Cpin_3070)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (35/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See A0A1X9Z6F7 at UniProt or InterPro

Protein Sequence (429 amino acids)

>CA265_RS14755 dihydrofolate synthase (Pedobacter sp. GW460-11-11-14-LB5)
MNYQQTLDFLYSKLPMFTRVGASAFKKDLTNTIILCKALDNPQDKFKSIHVAGTNGKGST
SHMLASVLQAQGYKTGLYTSPHLKDFRERIRINGKMMSKTEVVSFVKDQQKLIEKTEPSF
FEVTVAMAFDHFAKHEVDVAVIEVGLGGRLDSTNIITPQISVITNISLDHMNMLGNTLAE
IASEKAGIIKKNIPVVIGETQEESAPVFINKAKSLAAPIVFADAVLTAKDFKIRNNKLSL
SVYQKGEIKYKNLQSDLTGVYQHKNILTVLETLAVLNEKTGIKTDQEAIYKGISQVKKQT
GLQGRWQTLAKNPLVICDTGHNEAGITEVIKNITQTNYQNLHIVFGMVKDKDISKVLSLM
PKNATYYFCKPDLERGLAADELKEQAATFNLIGNSYTSVAEAKEKAIQSADPKDLVFVGG
STFVVAEAI