Protein Info for CA265_RS13975 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: RNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 PF08032: SpoU_sub_bind" amino acids 26 to 89 (64 residues), 31 bits, see alignment E=2.6e-11 PF00588: SpoU_methylase" amino acids 106 to 240 (135 residues), 117.5 bits, see alignment E=5.5e-38

Best Hits

KEGG orthology group: K03437, RNA methyltransferase, TrmH family (inferred from 59% identity to psn:Pedsa_1362)

Predicted SEED Role

"tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)" (EC 2.1.1.34)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z6T0 at UniProt or InterPro

Protein Sequence (248 amino acids)

>CA265_RS13975 RNA methyltransferase (Pedobacter sp. GW460-11-11-14-LB5)
MLSKSQISFIKSLHQKKYRKEHGLFIVEGIKSIKEFFQSNYQIHTIFYNSEQYNLLPKLP
ANINLFEVKNAELDKISTLQTPQGFLALVYIPKNKELDLKALKNQFTLVLDGVQDPGNMG
TIIRTADWFGFKNIICSADCVEVFNPKTVQATMGSLARVNIYEADLPALLEKNTIPVFGA
LLDGESIYKTQWGTEGLVILGNEGKGISAEVIKKINKPVTIPRIGEAESLNVAVSAAIFC
AELVRVRN