Protein Info for CA265_RS12005 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: glutathione-dependent formaldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 44% identical to FADH_METMR: S-(hydroxymethyl)glutathione dehydrogenase (fdh) from Methylobacter marinus

KEGG orthology group: K00001, alcohol dehydrogenase [EC: 1.1.1.1] (inferred from 74% identity to chu:CHU_1246)

Predicted SEED Role

"Threonine dehydrogenase and related Zn-dependent dehydrogenases" in subsystem Threonine degradation

MetaCyc Pathways

3-methylbutanol biosynthesis (engineered) (6/7 steps found)
ethanol degradation II (3/3 steps found)
mixed acid fermentation (12/16 steps found)
pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
superpathway of anaerobic sucrose degradation (14/19 steps found)
acetaldehyde biosynthesis I (1/1 steps found)
superpathway of N-acetylneuraminate degradation (16/22 steps found)
phytol degradation (3/4 steps found)
heterolactic fermentation (13/18 steps found)
L-isoleucine degradation II (2/3 steps found)
L-leucine degradation III (2/3 steps found)
L-valine degradation II (2/3 steps found)
ethanol degradation I (1/2 steps found)
pyruvate fermentation to ethanol II (1/2 steps found)
(S)-propane-1,2-diol degradation (3/5 steps found)
acetylene degradation (anaerobic) (3/5 steps found)
ethanolamine utilization (3/5 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
salidroside biosynthesis (2/4 steps found)
L-methionine degradation III (1/3 steps found)
pyruvate fermentation to ethanol I (1/3 steps found)
pyruvate fermentation to ethanol III (1/3 steps found)
phenylethanol biosynthesis (2/5 steps found)
serotonin degradation (3/7 steps found)
superpathway of fermentation (Chlamydomonas reinhardtii) (4/9 steps found)
butanol and isobutanol biosynthesis (engineered) (2/8 steps found)
noradrenaline and adrenaline degradation (4/13 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z5D1 at UniProt or InterPro

Protein Sequence (385 amino acids)

>CA265_RS12005 glutathione-dependent formaldehyde dehydrogenase (Pedobacter sp. GW460-11-11-14-LB5)
MKAAVFHKPGDIRVDYVPDPKILDPRDVILKVTSTAICGSDLHILSGAVPQKDPLIMGHE
FMGIVEEVGASITNLKRGDRVVVPFPISCGKCFFCTHEASPACETSNFKNYGPNGDMMSQ
KGAALFGYTDLYGGYSGGQAQYVRVPYADISPRIVPEHLSDEQALFLTDIFPTGWSAIDW
AQLKGGEVVAIFGSGPVGLMAQKAAWINGASRVIAIDPLDYRLEKAKAVNNVDILNPHKV
DVVEAIREMTGGRGADLCVDAVGFEPERSFFDKVKATVHFEKGSIKVLEMCFEAVRRMGT
VSIMGVYGSPYDNFPLFRIFDKGITIKQGQAPVLNYIDKLIGLVNEGKVVLDDIITHTLP
LEDAAHGYKIFDNKEEDCVKVVLKP