Protein Info for CA265_RS10930 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 PF17764: PriA_3primeBD" amino acids 18 to 115 (98 residues), 89.6 bits, see alignment E=2.9e-29 PF04851: ResIII" amino acids 288 to 453 (166 residues), 53.5 bits, see alignment E=8.4e-18 PF00270: DEAD" amino acids 293 to 456 (164 residues), 66.8 bits, see alignment E=6.1e-22 TIGR00595: primosomal protein N'" amino acids 312 to 826 (515 residues), 590.1 bits, see alignment E=1.7e-181 PF18319: PriA_CRR" amino acids 542 to 567 (26 residues), 45.4 bits, see alignment (E = 1.8e-15) PF00271: Helicase_C" amino acids 599 to 685 (87 residues), 34.8 bits, see alignment E=5.1e-12 PF18074: PriA_C" amino acids 729 to 825 (97 residues), 49.8 bits, see alignment E=1.5e-16

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 71% identity to phe:Phep_1550)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z4L7 at UniProt or InterPro

Protein Sequence (826 amino acids)

>CA265_RS10930 primosomal protein N' (Pedobacter sp. GW460-11-11-14-LB5)
MNTFESDIFAERETLFVEVILPLSLAKNYTYRVPFDLNDQIAVGKRVVVQFGKHKIYTAL
ISGISTVPPTVYEAKYIIDVVDTEPVITPTQLKFWTWMTNYYMCNEGDVMAAALPASLKL
ASETILILREDYNEDTELTDKEEIIINALKQQQKLTVNDVSKLLGQKTVYPIINHLLDKE
LVLVAEEVVQKYKPLLKSFIILNDFYSDEENLKQLFNVLDRAPKQLDALLAYLKLQKSNL
PISKEQLLEESNCGPAALKALTDKDIFVVMKRPVSRLAAHDEEFSVNFELNEGQQKALGQ
INQYFEEKEVVLLHGVTASGKTQVYIKLIEKIIQNTDGQVLFLLPEIALTTQIVERIKRY
FGNSIGVYHSKFNNSERVEIWNKVRTGAYKVILGARSAVFLPFEHLKLIVVDEEHEPSYK
QYDPAPRYQARDAAIYLGYLHQAKVILGSATPSLESYYNALQGKYGLVEMKERFGGVQLP
NQQVVSISEETKKKTMSSYFSSVLIKDIDLALSKKEQIVLFQNRRGYATILICATCGYTP
KCVNCDVSLTYHKSSGKLHCHYCGYQQSSVNICPACGSVHVEQKGFGTERIEEELRLLYP
EVTIARLDMDSTRTKNGLQQILNDFQEKKTDILIGTQMVAKGLDFDNLNLIGVINADTLL
GYPDFRAYERSFQLLAQVAGRAGRRADQGNVCIQTYDAENRIIKQVVNNDYEGMYNDEIV
EREKFLYPPFSRMIFLYIKHKDSHVLDHAAFTLANILKGKFGKRVLGPEQPLVSRVRNLY
IKQIIIKADKHTAIQKVKDALRETLTQFNATKEFKGVFTQIDVDPY